致畸试验固定液 (内脏检查)，主要由 PA 溶液、甲醛、冰乙酸复配而成，为黄色澄清液体，带有轻微刺激性气味。 作用原理：可快速固定胎仔内脏软组织，完整保存脑部、心、肺、肝、肾、生殖器等脏器原有形态与细微病变，防止组织自溶、萎缩，便于后续徒手切片开展内脏畸形筛查。 适用范围：用于食品、化妆品、保健品等毒理学安全性评价，专门用于致畸试验中胎仔内脏组织标本固定。
操作步骤 (仅供参考)：
1、样本取材分组 (大鼠、小鼠适用):
1) 每窝活胎平均分为两份，1/2 胎仔用于骨骼染色，剩余 1/2 胎仔用本品进行内脏固定。
2) 无需剥皮、剔除骨骼，完整胎仔直接投入固定液。
注：家兔致畸试验不可分半取样，全部胎仔均需同步固定处理。
2、内脏标本固定：
1) 将待检完整胎仔全部浸没于本品固定液中，密封避光室温浸泡固定不少于 14 天。
2) 固定期间容器密封，定期查看，确保标本完全浸没，防止组织裸露自溶。
3、固定后清洗预处理：
1) 固定满 14 天后取出胎仔，流水充分冲洗，去除标本表面黄色色素。
2) 冲洗完毕即可按照国标要求进行头部、胸腹部徒手切片，开展内脏畸形观察。
4、内脏镜检统计要求：
1) 沿标准 5 处切面依次观察嗅球、脑室、眼球、心脏、肺、肝、肾、生殖系统等脏器。
2) 记录无脑、小眼、室间隔缺损、膈疝、肾积水、脏器缺失/异位等各类内脏畸形。
3) 所有内脏畸形指标均以窝为单位进行统计，计算内脏畸形率。
注意事项：
1、该试剂仅用于科研领域，不适用于临床诊断或其他用途。
2、固定时长不得少于 14 天，固定不足会导致内脏组织软烂，无法清晰分辨畸形病变。
3、仅用于胎仔内脏组织固定，不可用于骨骼标本前期固定。
4、整套试验配套方案：每窝胎仔分半， 一半采用致畸试验固定液(骨骼检查)固定做骨骼染色，一半本品固定做内脏切片，同步完成骨骼 + 内脏致畸双重评价。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Teratogenicity‑Test Fixative (Visceral Examination) is mainly composed of PA solution, formaldehyde and glacial acetic acid. It is a yellow‑clear liquid with a slight pungent odour.Principle of Action: It can rapidly fix the visceral soft tissues of fetuses, completely preserve the original morphology and subtle lesions of organs including the brain, heart, lungs, liver, kidney and genital organs, prevent tissue autolysis and atrophy, and facilitate subsequent free‑hand sectioning for visceral malformation screening.Scope of Application: It is applied to the toxicological safety evaluation of food, cosmetics, health‑care products and other products, and specially used for the fixation of fetal visceral tissue specimens in teratogenicity tests.
Operating Procedure (For Reference Only):
1. Sampling and Grouping (Applicable to Rats and Mice)
1) Divide the live fetuses of each litter equally into two parts. One‑half of the fetuses are used for skeletal staining, and the remaining half are fixed with this product for visceral examination.
2) No skinning or bone removal is required. Place the intact fetus directly into the fixative solution.
Note: Half‑sampling is not allowed for rabbit teratogenicity tests. All fetuses shall be fixed synchronously.
2. Fixation of Visceral Specimens:
1) Fully submerge all intact fetuses to be tested in this fixative. Seal the container, protect it from light, and incubate at room temperature for no less than 14 days.
2) Keep the container sealed during fixation. Inspect specimens periodically to ensure complete submersion and avoid autolysis of exposed tissues.
3. Washing Pretreatment after Fixation:
1) After 14‑day fixation, take out the fetuses and rinse thoroughly under running water to remove yellow pigments on the specimen surface.
2) After rinsing, perform free‑hand sections of the head, thorax and abdomen in accordance with national standards for visceral malformation observation.
4. Statistical Requirements for Visceral Microscopic Examination:
1) Observe the olfactory bulb, cerebral ventricle, eyeball, heart, lung, liver, kidney, reproductive system and other organs sequentially along 5 standard cutting planes.
2) Record all types of visceral malformations, such as anencephaly, microphthalmia, ventricular septal defect, diaphragmatic hernia, hydronephrosis, organ absence / ectopia, etc.
3) All visceral malformation indicators shall be counted on a litter‑basis to calculate the visceral malformation rate.
Precautions:
1. This reagent is intended for research‑use only. It is not suitable for clinical diagnosis or other purposes.
2. The fixation period shall not be shorter than 14 days. Insufficient fixation will soften visceral tissues and impair the identification of malformed lesions.
3. For fetal visceral tissue fixation only. Not for pre‑fixation of skeletal specimens.
4. Recommended test scheme: Divide fetuses from each litter into two subgroups. One subgroup is fixed with Teratogenicity‑Test Fixative (Skeletal Examination) for skeletal staining; the other subgroup is fixed with this product for visceral sectioning, so as to complete dual evaluation of skeletal and visceral teratogenicity simultaneously.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
危险品化学品经营许可证（带存储）