计算溶液所需的质量、体积或浓度。
组织细胞固定液 (1% PFA)
有货
为什么选择此级别
BioReagent,即用型 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
多聚甲醛(Paraformaldehyde，简称PFA)是免疫组织化学(IHC)和免疫细胞化学(ICC)研究中最常用的固定剂之一。低聚合度多聚甲醛代替普通工业甲醛水溶液，在合成农药、合成树脂、涂料及制取熏蒸消毒剂等多种多样的甲醛下游产品中，既可减少脱水的能耗，又可大大减少废水处理量，这是一项利国利民绿色环保工程。
低聚合度多聚甲醛因其较工业甲醛有效成分高，是固体颗粒，有利于化工、制药等化学合成及其他工业领域的应用，特别是在要求使用无水甲醛作原料的合成方面，用途广泛。
阿拉丁生产的组织细胞固定液 (1% PFA)主要由多聚甲醛、磷酸盐、去离子水组成，pH 为 7.4，该固定剂较为温和，能很好地保存组织的抗原性和细微结构，适于组织标本和细胞片的较长期保存，非常适合组织和细胞的光镜免疫化学研究。
该产品仅适用于科研实验，不可做他用。
操作步骤（仅供参考）：
一般组织固定时间控制在 4～12 h，大标本应适当延长固定时间，培养细胞或细胞爬片 固定时间控制在，特殊情况除外。
注意事项：
1、 多聚甲醛溶液(1% PFA)有一定刺激性和腐蚀性。一经开启，储存过久固定效果易下降。
2、 避免过度延长固定时间，否则引起生物大分子过度交联。取材厚度不同，固定时间也不同。
3、 固定液的容量应足够，一般固定液与组织块的体积比率应大于 10:1。
4、 温度对固定的影响很明显，提高温度可以加速固定作用，但温度不宜过高。
5、 取出新鲜组织后，应及时固定，无法及时固定时，应保存于生理盐水中及时送检。
6、 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Paraformaldehyde (PFA) is one of the most commonly used fixatives in immunohistochemistry (IHC) and immunocytochemistry (ICC) research. Low-polymerization-degree paraformaldehyde, used as a substitute for conventional industrial formaldehyde aqueous solutions, offers significant advantages in the synthesis of pesticides, synthetic resins, coatings, and the production of fumigation disinfectants, as well as various other downstream formaldehyde products. It not only reduces energy consumption during dehydration but also greatly minimizes wastewater treatment volume, representing an environmentally friendly and socially beneficial green engineering solution.
Low-polymerization-degree paraformaldehyde, due to its higher active ingredient content compared to industrial formaldehyde and its solid particulate form, is advantageous for chemical synthesis, pharmaceutical manufacturing, and other industrial applications, particularly in syntheses requiring anhydrous formaldehyde as a raw material, where it finds extensive use.
The Tissue-cell Fixative (1% PFA) produced by Aladdin is primarily composed of paraformaldehyde, phosphate salts, and deionized water, with a pH of 7.4. This fixative is relatively mild, effectively preserving tissue antigenicity and fine structure. It is suitable for long-term preservation of tissue specimens and cell slides, making it highly appropriate for light microscopic immunochemical studies of tissues and cells.
This product is intended for research use only and shall not be used for other purposes.
Operating Procedure (For Reference Only):
Generally, tissue fixation time should be controlled at 4–12 hours. Larger specimens should have the fixation time appropriately extended. For cultured cells or cell culture slides, the fixation time should be controlled accordingly, except under special circumstances.
Precautions:
1.Paraformaldehyde solution (1% PFA) is somewhat irritating and corrosive. Once opened, the fixative effectiveness may decrease with prolonged storage.
2.Avoid excessively prolonging the fixation time, as this may cause excessive cross-linking of biological macromolecules. Fixation time varies depending on the thickness of the tissue samples.
3.The volume of fixative should be sufficient; generally, the volume ratio of fixative to tissue block should be greater than 10:1.
4.Temperature significantly affects fixation. Increasing the temperature can accelerate the fixation process, but the temperature should not be too high.
5.Freshly excised tissues should be fixed promptly. If immediate fixation is not possible, they should be stored in physiological saline and submitted for testing as soon as possible.
6.For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
规格或纯度
BioReagent, 即用型
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
英文名称
Tissue‑Cell Fixative (1% PFA)
储存条件
2-8°C储存,避光
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
产品属性
| pH | 7.4 |
|---|
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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