计算溶液所需的质量、体积或浓度。
Gram-Weigert 革兰染色液 (苯胺结晶紫法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
革兰氏染色法是丹麦医生 Christain Gram 于 1884 年所发明，是细菌学中广泛使用的种鉴别染色法，亦是一种复染法，通过此法染色可将细菌鉴别为革兰阳性菌(G )和革兰阴性菌(G)两大类。细菌的不同显色反应是由于细胞壁对乙醇的通透性和抗脱色能力的差异主要是肤聚糖层厚度和结构决定的，在同样染色环境中利用细菌不同的等电点(Gram 阳性细菌等电点为 pH2.0~3.0，Gram 阴性细菌等电点为 pH4.0~5.0)，阳性细菌带的负电荷比阴性细菌带的负电荷多，与带正电荷的碱性染料如结晶紫结合较牢，再加入媒染剂(碘)进入菌体后，与染料结合形成不溶于水的结晶紫-碘-蛋白复合物，并与阳性菌菌体内的核糖核酸镁盐结合，使已着色的细菌不易脱色;而分化剂(苯胺、丙酮等)不易透过阳性菌的细胞壁故阳性菌不易退色;但分化剂容易进入阴性菌菌体内，溶解染料和碘复合物，使阴性菌脱色。
阿拉丁Gram-Weigert 革兰染色液 (苯胺结晶紫法)是在经典的革兰染色配方进行改进，使用苏木素和 Weigert 确液加强染色，该法也是在草酸按结晶紫法演化过来的;临床标本直接涂片,背景干净,胞内吞噬体清晰易辨认细菌染色特征典型，可以区分 Gram阳性细菌和阴性细菌，尤其适用于鉴别细菌和非细菌的蓝色微颗粒状物质(如钙盐)。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
组分与储存
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自备材料：
1、10%福尔马林固定液
2、温箱或水浴锅
3、显微镜
操作步骤（仅供参考）：
1、组织固定于 10%福尔马林固定液中，常规脱水包埋。
2、切片厚4~ 5 μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、苏木素染色液复染胞核 3~5min，倾去染液，流水冲洗 10min。
4、入伊红染色液加盖 56°C浸染 5~10min，倾去染液，稍水洗
5、苯胺结晶紫染色液滴染于切片上 5min，倾去染液，用滤纸稍吸干切片周围余液
6、Weigert 碘液滴染于切片上2~3min，倾去碘液，用滤纸反复吸干切片上的水分
7、用苯胺二甲苯溶液(1:2)分化，并不时轻轻摇动，至切片无紫色脱出，立即用二甲苯或脱蜡透明液洗涤，并在镜下观察。
8、如果分化不够可再次滴加苯胺分化液，直至切片上革兰阳性细菌显示清楚为止.
9、滴入新的二甲苯或脱蜡透明液反复洗涤多次，彻底把苯胺洗去，中性树胶封固
染色结果：
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注意事项：
1、经Weigert 碘液和水洗后，必须滤纸反复吸干切片上的水分，再滴加苯胺二甲苯溶液(1:2)，否则可能导致分化不均匀。
2、经二甲苯冲洗后，应在镜下观察。如分化不足，可再滴入苯胺二甲苯溶液(1:2)继续分化至阳性菌清晰为止，但注意不要分化过度。
3、最后用二甲苯或脱蜡透明液反复洗涤切片，把苯胺彻底清除，切片若残留少量苯胺，以后就容易褪色。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
The Gram staining method, developed by the Danish physician Christian Gram in 1884, is a widely used differential staining technique in bacteriology. Through this staining method, bacteria can be classified into two major groups: Gram-positive bacteria (G⁺) and Gram-negative bacteria (G⁻). The differential color reaction of bacteria is primarily attributed to the differences in cell wall permeability to ethanol and resistance to decolorization, which are mainly determined by the thickness and structure of the peptidoglycan layer. Under the same staining conditions, utilizing the different isoelectric points of bacteria (Gram-positive bacteria have an isoelectric point of pH 2.0–3.0, while Gram-negative bacteria have an isoelectric point of pH 4.0–5.0), Gram-positive bacteria carry more negative charges than Gram-negative bacteria and thus bind more firmly to positively charged basic dyes such as crystal violet. Upon addition of a mordant (iodine), which enters the bacterial cell, it combines with the dye to form a water-insoluble crystal violet-iodine-protein complex. This complex further binds with magnesium ribonuclease within Gram-positive bacteria, making the stained bacteria resistant to decolorization. The differentiating agents (aniline, acetone, etc.) cannot easily penetrate the cell wall of Gram-positive bacteria, thus Gram-positive bacteria are not readily decolorized; however, these differentiating agents readily enter Gram-negative bacteria, dissolving the dye-iodine complex and causing decolorization of Gram-negative bacteria.
The Aladdin Gram-Weigert Gram Staining Solution (Aniline Crystal Violet Method) is an improvement upon the classic Gram staining formulation, incorporating hematoxylin and Weigert's iodine solution to enhance the staining. This method has evolved from the ammonium oxalate crystal violet method. When applied to direct smears of clinical specimens, it produces a clean background with clearly visible intracellular phagocytic bodies, and the bacteria exhibit typical staining characteristics, allowing clear differentiation between Gram-positive and Gram-negative bacteria. It is particularly suitable for distinguishing bacteria from non-bacterial blue granular materials (such as calcium salts).
This reagent is intended for research use only and is not suitable for clinical diagnosis or other applications.
Contents & Storage
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Materials Required (User-Supplied):
1.10% Formalin fixative solution
2.Incubator or water bath
3.Microscope
Operating Procedure (For Reference Only):
1.Fix tissues in 10% formalin fixative solution, then perform routine dehydration and embedding.
2.Cut sections at 4–5 μm thickness. Deparaffinize through routine xylene or dewaxing clearing solution and rehydrate to water.
3.Counterstain the nuclei with Hematoxylin Staining Solution for 3–5 min. Discard the staining solution and rinse under running tap water for 10 min.
4.Immerse in Eosin Staining Solution, cover, and stain at 56°C for 5–10 min. Discard the staining solution and rinse briefly with water.
5.Apply Aniline-Crystal Violet Staining Solution dropwise onto the sections and stain for 5 min. Discard the staining solution and use filter paper to gently absorb the excess liquid around the sections.
6.Apply Weigert's Iodine Solution dropwise onto the sections and stain for 2–3 min. Discard the iodine solution and repeatedly absorb the moisture from the sections with filter paper.
7.Differentiate with aniline-xylene solution (1:2) while gently shaking occasionally, until no purple color elutes from the sections. Immediately wash with xylene or dewaxing clearing solution and examine under a microscope.
8.If differentiation is insufficient, apply additional aniline differentiation solution dropwise until the Gram-positive bacteria on the sections are clearly visible.
9.Wash repeatedly with fresh xylene or dewaxing clearing solution several times to thoroughly remove the aniline. Mount with neutral mounting medium.
Staining Results:
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Precautions:
1.After treatment with Weigert's Iodine Solution and water washing, the moisture on the sections must be repeatedly absorbed with filter paper before applying the aniline-xylene solution (1:2). Failure to do so may result in uneven differentiation.
2.After washing with xylene, examine under a microscope. If differentiation is insufficient, add additional aniline-xylene solution (1:2) dropwise and continue differentiation until the positive bacteria are clearly visible. However, take care to avoid over-differentiation.
3.Finally, wash the sections repeatedly with xylene or dewaxing clearing solution to completely remove the aniline. Residual aniline on the sections may cause subsequent fading.
4.For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
规格或纯度
BioReagent, 适用于微生物学, 用于显微镜, 生物染色剂
英文名称
Gram‑Weigert Stain (Aniline‑Crystal Violet Method)
应用
微生物染色
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at Room temperature long term (12 months). Store in the dark.
组分与储存
| Component No. | Component Name | Appearance | 4×10mL | 4×50mL | Storage |
| T1529298A | Hematoxylin Staining Solution | Liquid | 10mL | 50mL | Room temperature. Store in the dark. |
| T1529298B | Eosin Staining Solution | Liquid | 10mL | 50mL | Room temperature. Store in the dark. |
| T1529298C | Aniline-Crystal Violet Staining Solution | Liquid | 10mL | 50mL | Room temperature. Store in the dark. |
| T1529298D | Weigert's Iodine Solution | Liquid | 10mL | 50mL | Room temperature. Store in the dark. |
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