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Triton-NH₄OH细胞裂解液
有货
为什么选择此级别
BioReagent,无菌过滤,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
细胞的行为受基质调控，细胞在基质上附着、迁移和增殖，因此组织培养的表面包被胞外基质或单独的胞外基质组分可以更好的模拟细胞在体内生长的微环境，促进细胞的黏附增殖并表现出分化的能力。多种成分均可以从细胞中提取细胞外基质，如Triton、SDS、NP-40等。Triton-NH₄OH细胞裂解液是采用一种非变性裂解方法来温和裂解细胞，用于制备包括胶原、蛋白聚糖、层连蛋白、纤连蛋白、接触素、弹性纤维等在内的细胞外基质。
Triton-NH₄OH细胞裂解液（Triton-NH₄OH Lysis Buffer）主要由PBS、TritonX-100和NH₄OH等组成，经过除菌处理。Triton-NH₄OH Lysis Buffer处理过的细胞样品所得胞外基质组分可用于后续的细胞培养等实验。
该产品仅用于科研领域，不宜用于临床诊断或其他用途。
自备材料：
1、胰蛋白酶、胎牛血清/PBS、HBSS、生理盐水或无血清培养液
2、离心机、恒温箱或水浴锅
操作步骤（仅供参考）：
1、去除培养液：细胞经培养至1～5×10⁶个后，吸除培养液，PBS清洗一次。
2、裂解：取适量Triton-NH₄OH细胞裂解液提前37℃预热20 min，6孔板每孔加入1 mL Triton-NH₄OH细胞裂解液，轻柔吹打混匀，室温孵育5～10 min。
3、用相差显微镜检查细胞的裂解情况，如裂解完全，可以用2 mL PBS清洗各个孔。
4、如有必要，重复上述步骤一次。
注意事项：
1、操作过程中应注意无菌操作，尽量在超净工作台内操作。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用，以防影响后续实验效果。
he behavior of cells is regulated by the matrix. Cells attach, migrate, and proliferate on the matrix. Therefore, coating the surface of tissue culture vessels with extracellular matrix or individual extracellular matrix components can better mimic the microenvironment in which cells grow in vivo, promote cell adhesion and proliferation, and enable cells to exhibit differentiation capacity. Various components can be used to extract extracellular matrix from cells, such as Triton, SDS, NP-40, etc. Triton-NH₄OH Cell Lysis Buffer uses a non-denaturing lysis method to gently lyse cells, and is used to prepare extracellular matrix components including collagen, proteoglycans, laminin, fibronectin, contactin, elastic fibers, etc.
Triton-NH₄OH Cell Lysis Buffer is mainly composed of PBS, Triton X-100, and NH₄OH, etc., and has been sterilized. The extracellular matrix components obtained from cell samples treated with Triton-NH₄OH Lysis Buffer can be used for subsequent experiments such as cell culture.
This product is for research use only and is not suitable for clinical diagnosis or other purposes.
Materials Required but Not Provided:
1.Trypsin, fetal bovine serum/PBS, HBSS, normal saline, or serum-free culture medium
2.Centrifuge, incubator, or water bath
Procedure (for reference only):
1.Removal of culture medium: After cells have been cultured to 1–5×10⁶ cells, aspirate the culture medium and wash once with PBS.
2.Lysis: Prewarm an appropriate amount of Triton-NH₄OH Cell Lysis Buffer at 37 ℃ for 20 min in advance. Add 1 mL of Triton-NH₄OH Cell Lysis Buffer to each well of a 6-well plate, gently pipette up and down to mix, and incubate at room temperature for 5–10 min.
3.Examine the cell lysis condition using a phase-contrast microscope. If lysis is complete, wash each well with 2 mL PBS.
4.If necessary, repeat the above steps once.
Precautions:
1.Aseptic operation should be noted during the procedure, and operations should be carried out in a clean bench as much as possible.
2.For your safety and health, please wear a laboratory coat and disposable gloves during operation.
3.After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.
规格
规格或纯度
BioReagent, 无菌过滤, 用于细胞培养
稳定性与储存
Store at 2-8℃ long term (12 months).
英文名称
Triton‑NH₄OH Cell Lysis Buffer
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
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