计算溶液所需的质量、体积或浓度。
单组分TMB显色液 (超灵敏)
有货
为什么选择此级别
BioReagent,for ELISA 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品提供 ELISA 产品所需的全部底物溶液，主要成分为 TMB、H₂O₂以及稳定剂，具有检测灵敏度高的特点，开瓶即用，操作方便。与 HRP 反应生成的可溶性蓝色产物，可在 370 nm 和 655 nm处读取（652 nm 最合适）；添加酸性终止液后，产生的黄色产物可在 450 nm 处读数。
阿拉丁单组分TMB显色液系列有T117926、T1539125以及T1539126三款产品，在灵敏度等方面有所差异，本产品为三款中灵敏度最高的，可根据需要购买。
| 货号 | 产品描述 | 产品特点 | 适用范围 |
| T117926 | 单组分TMB显色液 | 1. 无色澄清液体 2. 灵敏度适中 3. 热稳定性优异 4. 耐受冻融，适配冬季、出口低温运输 | ELISA 常规实验，基础显色需求 |
| T1539125 | 单组分TMB显色液 (高灵敏) | 1. 无色澄清液体 2. 灵敏度高 3. 热稳定性优异 4. 耐受冻融，适配冬季、出口低温运输 | 对灵敏度有较高要求的 ELISA 检测 |
| T1539126 | 单组分TMB显色液 (超灵敏) | 1. 无色澄清液体 2. 超高灵敏度 3. 热稳定性优异 4. 耐受冻融，适配冬季、出口低温运输 5. 加终止液后信号可稳定 20 min | 高灵敏度 ELISA、低丰度样本检测 |
推荐使用方法：
1、 酶标板各检测孔添加 100~150 uL TMB单组分TMB显色液
2、 室温或 37℃ 孵育10-30 min
3、 向各个检测孔中加入 50~100 uL终止液
4、 将酶标板置于酶标仪中，450 nm 处读取结果
注意事项:
1、 用户需自备终止液
2、 完成添加终止液后，需在 5 min之内读取
3、 建议用户同时在 450 nm 以及 620~630 nm 处，开启双通道检测，获得更低本底检测结果。
4、 分装TMB，需使用棕色或者黑色等可避光的瓶子，并在使用之前，用超纯水清洗瓶子 2-3 次。
效果展示：
 结果表明：在标准 ELISA 检测体系下， Aladdin 单组分TMB显色液 (超灵敏) 的分析灵敏度与 Moss、Abcam 产品基本相当，且灵敏度优于参比品 Surmodics 和 Kementec |  结果表明：加入终止液 20 分钟后，Abcam 和 Moss 的 TMB 显色液出现了显著的信号衰减，尤其是在较高浓度水平（S4–S6）时。相比之下，Aladdin、Surmodics 和 Kementec 的 TMB 显色液则表现出优异的信号保持能力，且相对稳定 |
This product supplies the complete substrate solution required for ELISA assays. Its main components are TMB, H₂O₂ and stabilizers. It features high detection sensitivity and is ready-to-use directly after opening for convenient operation. The soluble blue product generated by reaction with HRP can be measured at 370 nm and 655 nm (652 nm is optimal). After adding acidic stop solution, the resulting yellow product can be read at 450 nm.
The Aladdin one-component TMB s ubstrate solution series includes three products: T117926, T1539125 and T1539126. These products differ in sensitivity and other performance indicators. This product delivers the highest sensitivity among the three variants and is available for purchase as required.
| Cat. No. | Product Name | Features | Application |
| T117926 | One‑Component TMB Substrate Solution | 1. Colorless clear liquid 2. Moderate sensitivity 3. Excellent thermal stability 4. Freeze‑thaw tolerant, suitable for low‑temperature shipment in winter and export logistics | Routine ELISA assays, basic chromogenic requirements |
| T1539125 | One‑Component TMB Substrate Solution (High Sensitivity) | 1. Colorless clear liquid 2. High sensitivity 3. Excellent thermal stability 4. Freeze‑thaw tolerant, suitable for low‑temperature shipment in winter and export logistics | ELISA tests requiring high sensitivity |
| T1539126 | One‑Component TMB Substrate Solution (Ultra‑Sensitive) | 1. Colorless clear liquid 2. Ultra‑high sensitivity 3. Excellent thermal stability 4. Freeze‑thaw tolerant, suitable for low‑temperature shipment in winter and export logistics 5. Signal remains stable for 20 min after stop solution addition | High‑sensitivity ELISA, detection of low‑abundance samples |
Recommended Protocol:
1. Add 100–150 μL one-component TMB chromogenic solution to each well of the microplate.
2. Incubate at room temperature or 37 ℃ for 10–30 min.
3. Add 50–100 μL stop solution into each well.
4. Place the microplate into a microplate reader and read the absorbance at 450 nm.
Precautions:
1. Stop solution needs to be prepared by the user.
2. Read the result within 5 min after adding stop solution.
3. Dual-wavelength detection at 450 nm and 620–630 nm is recommended to obtain results with lower background.
4. For aliquoting TMB, use brown or black light-shielding bottles. Rinse the bottles 2–3 times with ultrapure water before use.
Performance Demonstration:
Results indicated that, in a standard ELISA assay format, the analytical sensitivity of Aladdin One-Component TMB Substrate Solution (Ultra-high Sensitivity) was generally comparable to that of Moss and Abcam products, and its sensitivity was superior to that of the reference products from Surmodics and Kementec. | Results showed that 20 minutes after the addition of the stop solution, significant signal loss was observed in the TMB Substrate Solutions from Abcam and Moss, particularly at higher concentration levels (S4–S6). In contrast, the TMB Chromogenic Solutions from Aladdin, Surmodics, and Kementec demonstrated excellent signal retention and remained relatively stable. |
规格
别名
高灵敏度单组份 TMB 显色液 | 高灵敏度单组分 TMB ELISA 底物
英文别名
High Sensitivity One-Component TMB Substrate
规格或纯度
BioReagent, for ELISA, 过氧化物酶底物
稳定性与储存
Store at 2-8℃ long term (24 months). Store in the dark.
英文名称
One-Component TMB Substrate Solution (Ultra-Sensitivity)
储存条件
2-8°C储存,避光
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
light-sensitive
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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