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产品说明
本试剂提供了进行简便、灵敏的 PCR 检测系统。核心试剂包含了经过优化的缓冲液、dNTPs Mix、普通 Taq 酶(Taq DNA polymerase)和 Mg 2+(终浓度 2mM)溶液,使用者只需加入适量的引物和探针或荧光染料,即可进行荧光PCR 检测。
使用方法
使用前请注意混匀,然后按下列组份配制 50μl 反应体系的 PCR 反应液:
试剂 | 体积 | 终浓度 |
Taq PCR Mix(2×) | 25μl | 1× |
Primer-probe Mix | 3μl ※ | — |
模板 | 10μl ※ | — |
超纯水 | Up to 50μl | — |
总体积 | 50μl | — |
※注:引物、探针以及模板的用量可自行调整。
实验设置
步骤 | 温度 | 时间 | 循环数 |
1 | 95℃ | 2.5min | 1 |
2 | 94℃ | 15s |
40 |
3 | 55℃ | 40s(收集荧光) |
注:收集荧光设置为两步或者三步及条件可以根据您的实验设置自行设定。
Product manual
This reagent provides a simple and sensitive PCR detection system. The core reagent contains optimized buffer, dNTPs Mix, common Taq enzyme (Taq DNA polymerase) and Mg 2+ (final concentration 2mM) solution. Users only need to add appropriate primers and probes or fluorescent dyes to proceed. Fluorescence PCR detection.
Instructions
Please pay attention to mixing before use, and then prepare a 50μl PCR reaction solution of the reaction system according to the following components
Reagent | Volume | Final concentration |
Taq PCR Mix(2×) | 25μl | 1× |
Primer-probe Mix | 3μl | — |
Template | 10μl | — |
Ultra-pure water | Up to 50μl | — |
Total capacity | 50μl | — |
Note: The amount of primers, probes and templates can be adjusted by yourself.
Experimental setup
Step | Temperature | Time | Number of cycles |
1 | 95℃ | 2.5min | 1 |
2 | 94℃ | 15s |
40 |
3 | 55℃ | 40s |
Note: The collection of fluorescence is set to two or three steps and the conditions can be set according to your experimental settings.