C2626055计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.
TMB EASE characteristics are:
1:1 replacement of carcinogenic 2mg OPD
Aqueous based – no organic solvents (REACH Annex XVII compliant)
Low lot-to-lot variation
Product Characteristics
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.
TMB EASE is a ready-to-use and stable aqueous formulation. This substrate gives a slow assay reaction and is highly suitable for the direct switch from OPD (ortho-phenylenediamine dichloride) based assays. It does not contain NMP (1-methyl-2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use.
Composition & Properties
Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.
Working Procedure
1. The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use.
2. A recommended volume of 100 µl substrate solution per microtiter well is added. Time of development is typically 5-30 minutes. Keep the plate in the dark during incubation.
3. In kinetic assays, the blue color is read directly at 650 nm. For endpoint assays 100 µl (equal volumes) of 0.20 M sulphuric acid is added and the absorbance is read at 450/620 nm. The yellow solution is stable for at least one hour.
Tips & Tricks
• If reduced intensity is required, it is recommended to shorten the time of development or to dilute the conjugate/antibody in the immunoassay further.
• Dilution of the substrate is not recommended.
• In case of incidents of precipitation with very high OD-values, we can recommend the use of the TMB X-tnd (cat. no. T494100).
Handling & Storage
• Store solution at 2-8 °C in the dark.
• Avoid exposure to light, heat and contamination with metal ions or peroxidase.
• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color.
• Dispensing guidelines are available upon request.
规格
规格或纯度
Ready-to-use (RTU)
英文名称
TMB EASE
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
图片
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
溶液计算器
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