E2627144计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.
TMB X-tnd is developed to support assays, which require low detection limits while supporting OD readings of highly positive samples – with no precipitation of the TMB substrate, at up to 5.8 mAbs within 60 minutes.
TMB X-tnd characteristics are
Best dynamic range
No precipitation
Aqueous based – no organic solvents (REACH Annex XVII compliant)
Product Characteristics
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.
TMB X-tnd is a ready-to-use, very active and stable aqueous formulation. TMB X-tnd is developed to support assays, which require low detection limits while supporting OD readings of highly positive samples – with no precipitation of the TMB substrate, at up to 5.8 mAbs within 60 minutes. It does not contain NMP (1-methyl-2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use.
Composition & Properties
Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.
Working Procedure
1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use.
2.A recommended volume of 100 µL substrate solution per microtiter well is added.
3.In kinetic assays, the blue color is read directly at 650 nm. For endpoint assays 100 µL (equal volumes) of 0.35 M sulphuric acid is added and the absorbance is read at 450/620 nm. The yellow solution is stable for at least one hour.
Tips & Tricks
• Dilution of the substrate is not recommended unless a corresponding TMB X-tnd Diluent is acquired. The diluted TMB solution maintains the same stability performance as the undiluted version, and a specific activity can be achieved.
• If reduced intensity is required it is recommended to shorten the time of development or to dilute the conjugate/antibody in the immunoassay further.
Handling & Storage
• Store solution at 2-8 °C in the dark.
• Avoid exposure to light, heat and contamination with metal ions or peroxidase
• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.
规格
规格或纯度
Ready-to-use (RTU)
英文名称
TMB X-tnd
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
图片
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
溶液计算器
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