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G23121113计算溶液所需的质量、体积或浓度。
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基本描述
| 别名 | TMB | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 规格或纯度 | Ready-to-use (RTU) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 英文名称 | TMB X-tra | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 储存温度 | 2-8°C储存,避光 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 冰袋运输 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
产品规格与参数
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride) TMB X-tra characteristics are: High sensitivity Stable and robust Aqueous based – no organic solvents (REACH Annex XVII compliant) Low lot-to-lot variation For traceable pipetting, we recommend using pink colored TMB X-tra Prestained (cat. no. T494156-01). The pink indicator disappears after addition of the stop solution. Product Characteristics TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm TMB X-tra is a ready-to-use, very active and stable aqueous formulation. The substrate gives a very high signal for a particularly fast assay reaction. The composition of the product makes it very resistant to raised temperatures. It does not contain NMP (1-methyl-2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use. Composition & Properties Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added. Working Procedure 1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use. 2.A recommended volume of 100µL substrate solution per microtiter well is added. Time of development is typically 5-30 minutes. Keep the plate in the dark during incubation. 3.In kinetic assays, the blue color is read directly at 650 nm. For endpoint assays 100 µl (equal volumes) of 0.20 M sulphuric acid is added and the absorbance is read at 450/620 nm. The yellow solution is stable for at least one hour. Tips & Tricks • If reduced intensity is required, it is recommended to shorten the time of development or to dilute the conjugate/antibody in the immunoassay further. • Dilution of the substrate is not recommended unless a corresponding TMB X-tra Diluent is acquired. The diluted TMB solution maintains the same stability performance as the undiluted version, and a specific activity can be achieved. • For traceable pipetting, we recommend using the pink colored TMB X-tra Prestained (cat. no. T494156-01). The pink indicator disappears after addition of the stop solution. • In case of incidents of precipitation with very high OD-values, we recommend the use of the TMB X-tnd (cat. no. T494100) Handling & Storage • Store solution at 2-8 °C in the dark. • Avoid exposure to light, heat and contamination with metal ions or peroxidase. • Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request. Product specifications and parameters
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