F23061939计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.
TMB Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.
The substrate does not contain NMP (1-methyl-2pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.
Product Characteristics
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.
TMB Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.
The substrate does not contain NMP (1-methyl-2- pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use.
Composition & Properties
Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.
Working Procedure
The following procedure is applicable to nitrocellulose membranes. The procedure must be optimized for other membranes.
1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use.
2.In order to prevent the blotted membrane from fading, we recommend the use of 0.1M K2HPO4, pH 7.2 in coating.
3.After the last incubation with HRP-labelled Streptavidin or HRP-labelled secondary antibody, it is recommended to wash the membrane in a 0.1 M Tris buffer pH 7.4.
4.Shake off the excess buffer and incubate the membrane in the TMB Blotting solution for 10 minutes.
5.Wash the membrane in distilled water and allow it to dry.
6.The site of positive reaction will appear light blue with no or very little background staining.
Tips & Tricks
• The membrane can be blocked, prior to applying the substrate, with Kementec’s Synthetic Blocking Buffer for Blotting (cat. no. S494457).
• For long-term preservation of the results, the membranes must be stored in the dark.
Handling & Storage
• Store solution at 2-8 °C in the dark.
• Avoid exposure to light, heat and contamination with metal ions or peroxidase
• Re-dispense only into bottles made of High-Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.
规格
别名
TMB 印迹
规格或纯度
Ready-to-use (RTU)
英文名称
TMB Blotting
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
常见问题
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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