计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.
TMB-D Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.
The substrate does not contain NMP (1-methyl2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.
Product Characteristics
TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP.
TMB-D Blotting liquid ready-to-use substrate is a highly active and stable blotting substrate utilized for measuring HRP probe activity. A stable blue precipitate is formed at the reaction site.
The substrate does not contain NMP (1-methyl-2- pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal waste problems after use.
Composition & Properties
Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.
Working Procedure
The following procedure is applicable to nitrocellulose membranes. The procedure must be optimized for other membranes.
1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature, in the dark, before use.
2.After the last incubation with HRP-labelled Streptavidin or HRP-labelled secondary antibody it is recommended to wash the membrane in a 0.1 M Tris buffer pH 7.4.
3.Shake off the excess buffer and incubate the membrane in the TMB-D Blotting solution for 10 minutes.
4.Wash the membrane in distilled water and allow it to dry.
5.The site of positive reaction will appear light blue with no or very little background staining.
Tips & Tricks
• The membrane can be blocked with Kementec’s Synthetic Blocking Buffer for Blotting, (cat. no. S494457).
• For long-term preservation of the results, the membranes must be stored in the dark.
Handling & Storage
• Store solution at 2-8⁰C in the dark.
• Avoid exposure to light, heat and contamination with metal ions or peroxidase.
• Re-dispense only into bottles made of High-Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.
规格
别名
TMB-D印迹
规格或纯度
Ready-to-use (RTU)
英文名称
TMB-D Blotting
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
小分子
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 F23061936 |
分析证书 | T494241 |
| F23061953 |
分析证书 | T494241 |
| F23062012 |
分析证书 | T494241 |
| F23061933 |
分析证书 | T494241 |
| F23061976 |
分析证书 | T494241 |
| F23061979 |
分析证书 | T494241 |
| C2617150 |
分析证书 | T494241 |
| F23061935 |
分析证书 | T494241 |
| F23061946 |
分析证书 | T494241 |
| F23061984 |
分析证书 | T494241 |
溶液计算器
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