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概述
阿拉丁的UltraBio™ TBE PAGE预制胶(UltraBio™ TBE Precast PAGE Gel)是一种使用安全、便捷、高品质的常规尺寸聚丙烯酰胺非变性预制凝胶，主要用于10-3000bp双链核酸或10-3000nt单链核酸的高分辨率电泳分析或纯化，广泛用于限制性酶切产物、PCR产物、Southern和引物分析等的实验。本预制胶有1.5厘米高的浓缩胶，具有非常优良的分离效果，电泳后DNA条带平整、清晰、细腻、锐利，几乎没有边缘效应；本预制胶胶板为玻璃材质，电泳效果非常好，达到甚至超过了自配TBE PAGE胶的电泳效果。
阿拉丁的UltraBio™ TBE PAGE预制胶提供不同浓度的梯度胶和固定浓度胶，并有10孔和15孔两种孔数选择。固定浓度胶包括5%、10%、12%和15%；梯度胶浓度为4-20%。
每种预制胶的最佳分离范围请参考下表：
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本预制胶含有1.5厘米高的4%浓缩胶，可以有效确保获得非常锐利的条带。
本预制胶丙烯酰胺(Acrylamide)与甲叉丙烯酰胺(Bisacrylamide)的比例为29:1，凝胶厚度为1.5mm。加样孔数为10孔的最大上样量为60μl，加样孔数为15孔的最大上样量为30μl。胶板尺寸：宽×高×厚度为98×84×4.1mm；凝胶尺寸：宽×高×厚度为81×74×1.5mm。
聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis, PAGE)技术广泛用于蛋白质、核酸及蛋白质-核酸复合物的分离纯化、检测、鉴定、分子量分析等实验，是生命科学研究中最基本的实验技术之一。常见的Western印迹(Western blot)检测就是基于PAGE的。
核酸电泳通常是基于长度来电泳分离DNA或RNA片段的分析技术。将待分析的核酸样品置于凝胶中，核酸由于其糖磷酸主链带负电在电场的作用下向阳极迁移。不同大小的片段能够通过在凝胶中的迁移速度差异来完成分离，较长的分子迁移速度较慢，因为它们在凝胶中的阻力较大。由于分子的大小影响其迁移速度，在一定的时间内，较短的片段比较长的片段更接近阳极。在核酸电泳实验中，常用的有两种凝胶：琼脂糖凝胶和聚丙烯酰胺凝胶。与琼脂糖凝胶相比，聚丙烯酰胺凝胶具有更高分辨率和灵敏度、低本底染色、所需样品量较少、高效印迹、易于从凝胶中提取DNA、不干扰酶反应以及准确性和可重复性高等优点。常用于核酸电泳的PAGE凝胶有TBE PAGE凝胶、TBE-Urea PAGE凝胶和EMSA PAGE凝胶等。其中，TBE PAGE凝胶由TBE缓冲液(Tris-硼酸-EDTA)制备，主要用于核酸的非变性电泳，常应用于DNA或RNA片段的高分辨率分析，包括限制性酶切产物、PCR产物、Southern和引物等的分析等实验。
阿拉丁的UltraBio™ TBE PAGE预制胶 预制胶由高纯度的Tris、硼酸、EDTA等试剂制备，缓冲能力强，适合长时间电泳，且分辨率高、分离效果佳。严格的质量控制可确保凝胶和缓冲液中无DNase和RNase。
关于10孔和15孔预制胶的选择：需要检测的样品数量多或者需要定量时，推荐使用15孔预制胶，通量更大、更便于进行较多样品的定量统计分析；需要获得非常漂亮的代表性图片时，推荐使用10孔预制胶，10孔预制胶获得的条带更加平整和锐利。
本产品使用安全、便捷。本预制胶无需配制，即开即用，去掉梳子即可上样，而传统的TBE PAGE配制凝胶繁琐费时，并且制胶时还会接触有毒和刺激性试剂。
本产品质量稳定。本预制胶采用高品质玻璃胶板，和塑料胶板相比，大大减少了胶板对核酸的吸附，电泳效果更好。本产品流水线灌注，品质稳定可靠，重复性好，不同批次的产品一致性高。
本产品电泳效果好。本预制胶的核酸分离效果极佳，核酸条带平整、清晰、细腻、锐利。
本产品电泳时间短。本预制胶推荐的电泳电压和电泳时间为150V 50-75分钟，即可完成电泳并获得非常平整和锐利的电泳条带。具体的电泳时间取决于凝胶浓度。
本产品取出凝胶极为便捷。只需用刀片在玻璃胶板一侧轻轻划一下即可，并且玻璃胶板打开极为方便，无需特殊的起撬工具。
注意事项：
本预制胶不能置于0℃以下冷冻，否则凝胶会冻裂。
电泳液建议新鲜配制，试剂纯度不够、反复使用或长期放置的缓冲液会降低电泳效果。电泳缓冲液多次使用后，离子强度降低，pH值上升，缓冲性能下降，可能使电泳产生条带模糊和不规则的DNA带迁移的现象。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
Aladdin UltraBio™ TBE Precast PAGE Gel is a safe, ready-to-use, and high-quality gel with regular size. It is primarily used for high-resolution separation or purification of 10-3000bp double-stranded nucleic acids or 10-3000nt single-stranded nucleic acids, such as digested DNA with restriction enzymes, PCR products, primers, and RNA for Southern analysis. This precast gel has a stacking gel of 1.5cm in height. The nucleic acid bands after electrophoresis are flat, clear, fine, and sharp, with minimal edge effects. Additionally, this product employs the high-quality glass plates to reduce non-specific nucleic acid adsorptions, with better resolution effects than home-made TBE PAGE gels.
Aladdin provides a variety of UltraBio™ TBE Precast PAGE Gel at different concentrations. Gradient gel is available in concentration of 4-20%. Fixed gels include 5%, 10%, 12% and 15% gels. Please refer to the table below for parameters of UltraBio™ TBE Precast PAGE Gel.
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This precast gel contains a 4% stacking gel with a height of 1.5cm to ensure sharp nucleic acid bands can be obtained.
The ratio of acrylamide to bisacrylamide in this precast gel is 29:1, and the gel thickness is 1.5mm. The maximum loading volume per well of 10-well and 15-well gels is 60μl and 30μl, respectively. The gel plate size is 98×84×4.1mm (width × height × thickness) with a gel size of 81×74×1.5mm (width × height × thickness).
Polyacrylamide gel electrophoresis (PAGE) is a widely used technique in life science research for the separation, purification, assay, identification, and molecular weight analysis of proteins, nucleic acids, and protein-nucleic acid complexes. The commonly used Western blot technique is based on PAGE.
Nucleic acid electrophoresis is a commonly used approach for resolving DNA or RNA fragments based on length. The nucleic acids loaded into gel wells migrate toward the anode in the presence of an electric field due to their negatively charged sugar-phosphate backbones. Nucleic acid fragments of different sizes can be separated during electrophoresis as they migrate at different rate, with longer fragments migrating more slowly than smaller fragments, thus shorter fragments are closer to the anode than longer ones after electrophoresis. For nucleic acid electrophoresis, two types of gels are commonly used: agarose gels and polyacrylamide gels. Compared to agarose gels, polyacrylamide gels have the advantages of higher resolution and sensitivity, lower background staining, less sample volume required, efficient blotting, easier DNA extraction from gels, no interference with enzyme reactions, and higher accuracy and reproducibility. PAGE gels commonly used for nucleic acid electrophoresis include TBE PAGE gels, TBE-Urea PAGE gels and EMSA PAGE gels. Among them, TBE PAGE gels are prepared from TBE buffer (Tris-boric acid-EDTA), and are mainly used for native electrophoresis of DNA or RNA fragments.
This product is prepared with high-purity Tris, boric acid, EDTA and other reagents. It has strong buffering capacity and is suitable for long time electrophoresis, ensuring high resolution and good separation of nucleic acids. Strict quality control ensures that the gel and buffer are free of DNase and RNase.
Selection of 10-well and 15-well precast gels: Nucleic acid bands obtained from 10-well precast gels are flatter and sharper. However, when the number of samples to be examined is large or quantification of nucleic acids is required, we recommend using the 15-well precast gel which has greater throughput and is more convenient for statistical quantitation analysis
This product is safe and convenient to use. There is no need for cumbersome gel preparation and casting, thus avoiding contact with toxic and irritating reagents. After electrophoresis, the gel can be easily removed from the glass plates.
The quality of this product is high and stable, with high consistency between batches. It uses high-quality glass plates to reduce adsorption of nucleic acids significantly, resulting in better electrophoresis performance. This product is manufactured using a streamlined process, ensuring its high and stable quality.
This product provides excellent electrophoresis results. It offers excellent nucleic acid resolution. Smooth, clear, fine, and sharp nucleic bands can be obtained.
The electrophoresis time is short. The native electrophoresis can be completed in 50-75 minutes at 150V. The run time can vary depending on experimental requirements.
The gel is easy to remove from the glass plates. Users only need to light scrape one side of the glass plate with a blade, and the glass plates can be easily opened without the need for special lifting tools.
This product should not be stored below 0℃ to avoid freezing and cracking.
The electrophoresis buffer in the inner chamber of the electrophoresis tank and the transfer buffer should be freshly prepared. Low-quality buffers, recycled buffers, or buffers that have been kept for too long period of time will result in poor protein resolution and transfer.
This product is for R&D only. Not for drug, household, or other uses.
For your safety and health, please wear a lab coat and disposable gloves during the operation.
规格
稳定性与储存
4℃保存，1-2个月有效。切勿置于0℃以下冷冻。
英文名称
UltraBio™ TBE Precast PAGE Gel
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
小分子
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产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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