阿拉丁的 UltraBio™小分子量蛋白预制胶 (Tricine) 是一款安全、快捷、高性能的预制聚丙烯酰胺凝胶。常用于超低分子量蛋白或多肽的电泳实验。
产品特点：
⁕ 采用自动化灌胶生产技术，确保产品质量的高稳定性和重复性。
⁕ 超高分辨率，最小可分离 1.2kDa的分子。
⁕ 采用玻璃胶板，有效减少蛋白非特异性吸附，使蛋白条带更为敏锐，清晰。
⁕ 电泳时间短，在150V电压下，电泳90分钟即可完成。
⁕ 胶夹打开极为轻松，只需用刀片在胶夹一侧轻轻划一下即可打开。
⁕ 兼容Bio-Rad、Invitrogen、天能、君意东方、北京六一等主流mini电泳槽。
基本信息：
胶板尺寸：宽×高×厚度为100×84×4.6mm
孔数：10孔，15孔
凝胶尺寸：宽×高×厚度为81×74×1.5mm
凝胶厚度：1.5mm
最大上样量：60μL，30μL
Acr-Bis：29: 1
浓缩胶：4%，1.5 cm
包装：10片/盒
产品保存：
1. 2-8°C储存，保质期1个月。
2. 请勿置于0°C以下，凝胶在0°C以下会冻凝，产生气泡和裂纹，导致凝胶报废。
预制胶选择指导：
货号
| 浓度
| 孔数
| 最大上样量
| 电泳液
| 转膜液
| 分离范围
| 建议电压
|
T753562-A2-10EA
| 18%
| 10
| 60 μL
| Tris-Tricine
| Tris-Gly
| 1.2-40 kDa
| 150 V
|
T753562-B2-10EA
| 18%
| 15
| 30 μL
| Tris-Tricine
| Tris-Gly
| 1.2-40 kDa
| 150 V
|
使用说明：
1. 将UltraBio™小分子量蛋白预制胶 (Tricine) 从包装袋中取出，固定在电泳槽中，安装好电泳装置。
2. 变性电泳缓冲液的准备：Tris-Tricine-SDS 电泳缓冲液 (T1373499) 配制成1×，向电泳槽内槽中注满1× Tris-Tricine 电泳缓冲液，覆盖加样孔。外槽注入1× Tris-Tricine 电泳缓冲液，需至少加至1/3 液面，最高不可漫过胶板，然后缓慢拔出梳子。
注：也可使用阴、阳极电泳缓冲液，阳极缓冲液 (T751594) 加入外槽，阴极缓冲液 (T751595) 加入内槽。
3. 上样前使用移液器吸取电泳缓冲液轻轻吹打点样孔，以去除点样孔内残余的胶液。
4. 上样：将蛋白样品与 2× 小分子蛋白上样缓冲液1:1混合均匀，100°C加热3-5 min。在梳孔内加入适当浓度和体积的蛋白样品。注意枪头不要戳破凝胶，不要过度插入梳孔使胶板变形造成漏液。
5. 电泳条件：150V，90 min 左右，当溴酚蓝指示带电泳至胶板底部，或实验预定位置时，即可结束电泳。为了获得更好的小分子蛋白分辨率，建议在4℃或冰水浴中进行电泳。
6. 电泳结束，取出凝胶。用刀在侧边胶处，沿着两片玻璃的缝隙切开封胶材料，即可打开玻璃板，取胶时，需在凝胶和玻璃条之间，沿着玻璃条划一刀，防止取胶时，发生粘连使凝胶破碎 (使用美工刀时请注意安全!)。
拆胶：
1. 先沿侧边胶处简单划一刀 (或先将玻璃板侧边多余封胶材料去除）。
2. 用刀在侧边胶处，沿着玻璃板和玻璃条的缝隙切开封胶材料 (箭头处)，轻轻打开玻璃板。
3. 取胶时，需在凝胶和两侧玻璃条之间沿着玻璃条划一刀，防止取胶时发生粘连使凝胶破碎。
UltraBio™ gel迷你预制胶兼容的电泳槽：
UltraBio™ gel系列预制胶可以兼容大部分的miniSDS-PAGE电泳槽，包括
a.Bio-Rad Mini-PROTEAN (II/3/TetraSystem)。
b.Hoefer Mighty Small (SE250/SE260/SE280)。
c.Life Technology Novex Mini-Cell (请与阿拉丁特制挡板01配合使用)。
d.LifeMiniGelTank小型胶电泳槽 (请与阿拉丁特制挡板02配合使用)。
e.北京六一DYCZ-25E、DYCZ-24K、DYCZ-24KS、DYCZ-24KF。
f.君意东方JY-SCZ2+。
g.天能VE180。
或其它胶板宽度在10厘米的电泳槽。
在 Life 电泳槽中的应用：如有需要，请在订购本产品时告知
将挡板01置于凝胶的外侧 将挡板02置于凝胶的内侧
在 Bio-Rad 电泳槽中的应用：
Bio-Rad Mini-PROTEAN 系列电泳槽的U型密封条顶部有凸起结构，而阿拉丁 UltraBio™ 系列预制胶的短玻板是凹形结构，因此该部位是平的，电泳前需将具有凸起结构的密封条取出后反过来安装，是平滑面朝外，从而防止漏液（如下图所示）。
a. 将 Bio-Rad 电泳槽中的U型密封条（如图绿色部分）拉出，注意这时的密封条两端是有凸起的，凸起的这面为正面，无凸起的为反面。
b. 将密封条旋转180度 (正面朝里，反面朝外)，重新装回电泳槽中，注意把密封圈周边压实，防止发生漏液。
c. 放置好预制胶进行正常的电泳操作即可。
注意事项：
1. 在转膜过程中，增加转膜液中甲醇的含量有助于提高小分子蛋白的转膜效率。建议甲醇的浓度为20%-30%。
2. 蛋白分子量<20kDa时，建议选择孔径0.22μm的膜。
3. 仅限于专业人员的科学研究用，不得用于临床医学诊断及其他非合理用途。不得存放于普通住宅内。
4. 为了您的安全和健康，请穿戴好个人防护装备和实验服装进行操作。
Aladdin UltraBio™ Tricine Precast PAGE Gel for Small Molecular Weight Protein is a safe, rapid and high-performance precast polyacrylamide gel. It is commonly used for electrophoresis experiments of ultra-low molecular weight proteins or peptides.
Product Features:
⁕ Manufactured by automated gel casting technology to guarantee high stability and reproducibility of product quality.
⁕ Ultra-high resolution; capable of separating molecules as small as 1.2 kDa.
⁕ Glass gel plates are adopted to effectively reduce non-specific protein adsorption and yield sharper, clearer protein bands.
⁕ Short electrophoresis duration; electrophoresis can be completed within 90 min at 150 V.
⁕ The gel cassette can be opened effortlessly. Simply slice gently along one side of the cassette with a razor blade to open it.
⁕ Compatible with mainstream mini electrophoresis tanks including Bio-Rad, Invitrogen, Tanon, Junyi Dongfang and Beijing Liuyi.
Basic Information:
Gel Plate Dimensions: Width × Height × Thickness = 100 × 84 × 4.6 mm
Number of Wells: 10 wells, 15 wells
Gel Dimensions: Width × Height × Thickness = 81 × 74 × 1.5 mm
Gel Thickness: 1.5 mm
Maximum Sample Loading: 60 μL, 30 μL
Acr‑Bis Ratio: 29 : 1
Stacking Gel: 4%, 1.5 cm
Package: 10 gels per box
Product Storage:
1. Store at 2–8 °C; shelf life is 1 month.
2. Do not store below 0 °C. Gels will freeze below 0 °C, generating air bubbles and cracks and rendering the gel unusable.
Precast Gel Selection Guide:
T753562
| Concentration
| Wells
| Maximum Sample Loading Volume
| Running Buffer
| Transfer Buffer
| Separation Range
| Recommended Voltage
|
T753562-A2-10EA
| 18%
| 10
| 60 μL
| Tris-Tricine
| Tris-Gly
| 1.2-40 kDa
| 150 V
|
T753562-B2-10EA
| 18%
| 15
| 30 μL
| Tris-Tricine
| Tris-Gly
| 1.2-40 kDa
| 150 V
|
Instructions for Use:
1. Take the UltraBio™ Low Molecular Weight Protein Precast Gel (Tricine) out of the pouch, mount it into the electrophoresis tank, and assemble the electrophoresis setup.
2. Preparation of denaturing electrophoresis running buffer: Dilute Tris-Tricine-SDS running buffer (T1373499) to 1×. Fill the inner chamber of the electrophoresis tank with 1× Tris-Tricine running buffer to cover the sample wells. Add 1× Tris-Tricine running buffer to the outer chamber to at least 1/3 of the chamber height; the liquid level must not exceed the gel cassette. Slowly remove the comb.
Note: Separate anode and cathode running buffers may also be used. Add anode buffer (T751594) to the outer chamber and cathode buffer (T751595) to the inner chamber.
3. Before sample loading, pipette running buffer to gently rinse the sample wells to remove residual gel solution inside the wells.
4. Sample loading: Mix protein sample with 2× low molecular weight protein loading buffer at a 1:1 ratio, and heat at 100 °C for 3–5 min. Load protein sample of appropriate concentration and volume into the wells. Take care not to pierce the gel with the pipette tip, and avoid excessive insertion into the well which may deform the gel cassette and cause buffer leakage.
5. Electrophoresis conditions: 150 V for approximately 90 min. Terminate electrophoresis when the bromophenol blue tracking dye migrates to the bottom of the gel cassette or the predetermined position. For improved resolution of small molecular weight proteins, electrophoresis is recommended at 4 °C or in an ice-water bath.
6. After electrophoresis, remove the gel assembly. Use a blade to cut the sealing compound along the gap between the two glass plates on the side of the gel cassette to separate the glass plates. To retrieve the gel, run the blade between the gel and glass edge to prevent adhesion and gel breakage during gel removal (Exercise caution when using a utility knife!).
Gel Disassembly:
1. First, make a simple cut along the side gel (or remove excess gel-sealing material from the sides of the glass plate first);
2. Use a knife to cut through the gel-sealing material along the gap between the glass plate and the glass strip at the side gel (at the arrow), then gently open the glass plate;
3. When removing the gel, make a cut along the glass strips between the gel and the glass strips on both sides to prevent the gel from sticking and breaking during removal.
Electrophoresis Tanks Compatible with UltraBio™ Gel Mini Precast Gels
UltraBio™ Gel series precast gels are compatible with most mini SDS-PAGE electrophoresis tanks, including:
a. Bio-Rad Mini-PROTEAN (II/3/Tetra System)
b. Hoefer Mighty Small (SE250/SE260/SE280)
c. Life Technology Novex Mini-Cell (use with Aladdin custom cassette shield 01)
d. Life Mini Gel Tank (use with Aladdin custom cassette shield 02)
e. Beijing Liuyi DYCZ-25E, DYCZ-24K, DYCZ-24KS, DYCZ-24KF
f. Junyi-Dongfang JY-SCZ2+
g. Tanon VE180
or other electrophoresis tanks with a gel plate width of 10 cm.
Application in Life Electrophoresis Tanks: If required, please inform us when ordering this product
Place Baffle No. 01 on the outer side of the gel Place Baffle No. 02 on the inner side of the gel
Application in Bio-Rad Electrophoresis Tanks:
The top of the U-shaped sealing strip in the Bio-Rad Mini-PROTEAN series electrophoresis tanks has a raised structure, while the short glass plate of Aladdin’s UltraBio™ series precast gels has a concave structure—resulting in a flat surface at this location. Before electrophoresis, the sealing strip with the raised structure must be removed and reinstalled in reverse, with the smooth side facing outward, to prevent liquid leakage (as shown in the figure below).
a. Pull out the U-shaped sealing strip (the green part in the figure) from the Bio-Rad electrophoresis tank. Note that the two ends of the sealing strip have protrusions at this point: the side with protrusions is the front side, and the side without protrusions is the back side.
b. Rotate the sealing strip 180 degrees (with the front side facing inward and the back side facing outward) and reinstall it in the electrophoresis tank. Ensure the perimeter of the sealing ring is pressed firmly to prevent liquid leakage.
c. Place the precast gel and proceed with normal electrophoresis operations.
Precautions:
1. During membrane transfer, increasing the methanol content in transfer buffer helps improve the transfer efficiency of low-molecular-weight proteins. A methanol concentration of 20%–30% is recommended.
2. For proteins with molecular weight <20 kDa, a membrane with a pore size of 0.22 μm is recommended.
3. For research use by professionals only. Not for clinical medical diagnosis or other inappropriate applications. Do not store in residential premises.
4. For your safety and health, wear personal protective equipment and laboratory attire during operation.
ZJ26F1039878
危险品化学品经营许可证（带存储）