阿拉丁的 UltraBio™大分子量蛋白预制胶 (Tris-Acetate) 是一款安全、快捷、高性能的预制聚丙烯酰胺凝胶。常用于大分子量蛋白的分离检测。
产品特点：
⁕ 采用自动化灌胶生产技术，确保产品质量的高稳定性和重复性。
⁕ 有效分离40-500kDa的大分子蛋白。
⁕ 采用玻璃胶板，有效减少蛋白非特异性吸附，使蛋白条带更为敏锐，清晰。
⁕ 电泳时间短，在150V电压下，60分钟即可完成电泳。
⁕ 胶夹打开极为轻松，只需用刀片在胶夹一侧轻轻划一下即可打开。
⁕ 兼容Bio-Rad、Invitrogen、天能、君意东方、北京六一等主流mini电泳槽。
⁕ 提供均一胶和梯度胶（7%，3-8%）。也可以提供特殊浓度的定制服务。
基本信息：
胶板尺寸：宽×高×厚度为100×84×4.6mm
孔数：10孔，15孔
凝胶尺寸：宽×高×厚度为81×74×1.5mm
凝胶厚度：1.5mm
最大上样量：60μL，30μL
Acr-Bis：37.5: 1
浓缩胶：4%，1.5 cm
包装：10片/盒
产品运输和保存：
1. 常温运输，常温保存时应放置于阴凉处，避免温度剧烈变化和阳光直射。
2. 2-8°C储存，保质期2个月。
3. 请勿置于0°C以下，凝胶在0°C以下会冻凝，产生气泡和裂纹，导致凝胶报废。
预制胶选择指导：
货号
| 浓度
| 孔数
| 最大上样量
| 电泳液
| 转膜液 | 分离范围 | 建议电压
|
T753564-A1-10EA
| 7%
| 10
| 60 μl
| Tris-乙酸
| Tris-乙酸
| 35-300 kD
| 150 V
|
T753564-B1-10EA
| 7%
| 15
| 30 μl
| Tris-乙酸
| Tris-乙酸
| 35-300 kD
| 150 V
|
T753564-A2-10EA
| 3-8%
| 10
| 60 μl
| Tris-乙酸
| Tris-乙酸
| 45-400 kD
| 150 V
|
T753564-B2-10EA
| 3-8%
| 15
| 30 μl
| Tris-乙酸
| Tris-乙酸
| 45-400 kD
| 150 V
|
使用说明：
1. 将UltraBio™大分子量蛋白预制胶 (Tris-Acetate)从包装袋中取出，固定在电泳槽中，安装好电泳装置。
2. 向电泳槽中加入1xTris-Acetate-SDS 电泳缓冲液(T767965)。内槽加满电泳缓冲液，外槽电泳缓冲液需至少加至1/3液面，最高不可漫过胶板，然后缓慢拔出梳子。
3. 用移液器轻轻吹打加样孔，清除残余胶液。
4. 上样：将蛋白样品与大分子蛋白上样缓冲液，2x(S750310)进行1:1混合均匀，加热处理(100°C，5-10 min)。上样时注意枪头勿刺破凝胶或插入过深使胶板变形造成后续电泳时漏液。
5. 接通电源，恒压条件下跑胶(150V)，当溴酚蓝指示带电泳至胶板底部，或实验预定位置时，即可结束电泳。
6. 电泳结束，取出凝胶。用刀在侧边胶处，沿着两片玻璃的缝隙切开封胶材料，即可打开玻璃板，取胶时需在凝胶和玻璃条之间，沿着玻璃条划一刀，防止取胶时，发生粘连使凝胶破碎(使用美工刀时请注意安全)。
预制胶分离图谱：
迁移率图谱 电泳图
拆胶：
1. 先沿侧边胶处简单划一刀（或先将玻璃板侧边多余封胶材料去除）。
2. 用刀在侧边胶处，沿着玻璃板和玻璃条的缝隙切开封胶材料（箭头处），轻轻打开玻璃板。
3. 取胶时，需在凝胶和两侧玻璃条之间沿着玻璃条划一刀，防止取胶时发生粘连使凝胶破碎。
UltraBio™ gel迷你预制胶兼容的电泳槽：
UltraBio™ gel系列预制胶可以兼容大部分的miniSDS-PAGE电泳槽，包括
a.Bio-Rad Mini-PROTEAN(II/3/TetraSystem)。
b.Hoefer Mighty Small(SE250/SE260/SE280)。
c.Life Technology Novex Mini-Cell(请与阿拉丁特制挡板01配合使用)。
d.LifeMiniGelTank小型胶电泳槽(请与阿拉丁特制挡板02配合使用)。
e.北京六一DYCZ-25E、DYCZ-24K、DYCZ-24KS、DYCZ-24KF。
f.君意东方JY-SCZ2+。
g.天能VE180。
或其它胶板宽度在10厘米的电泳槽。
在 Life 电泳槽中的应用：如有需要，请在订购本产品时告知
将挡板01置于凝胶的外侧 将挡板02置于凝胶的内侧
在 Bio-Rad 电泳槽中的应用：
Bio-Rad Mini-PROTEAN 系列电泳槽的U型密封条顶部有凸起结构，而阿拉丁 UltraBio™ 系列预制胶的短玻板是凹形结构，因此该部位是平的，电泳前需将具有凸起结构的密封条取出后反过来安装，是平滑面朝外，从而防止漏液（如下图所示）。
a. 将 Bio-Rad 电泳槽中的U型密封条（如图绿色部分）拉出，注意这时的密封条两端是有凸起的，凸起的这面为正面，无凸起的为反面。
b. 将密封条旋转180度 (正面朝里，反面朝外)，重新装回电泳槽中，注意把密封圈周边压实，防止发生漏液。
c. 放置好预制胶进行正常的电泳操作即可。
注意事项：
1. 电泳缓冲液不建议重复使用。因为经过电泳之后，缓冲液中的离子强度、缓冲能力都发生了变化，不能确保电泳效果；在转膜过程中，增加转膜液中甲醇的含量有助于提高小分子蛋白的转膜效率。建议甲醇的浓度为20%-30%。
2. 甲醇可能导致大分子蛋白沉淀。为了避免这种情况，可以将转膜液中甲醇的浓度减少至5%。为进一步确保蛋白不沉淀，可以添加SDS至终浓度为0.1%。SDS为蛋白提供均匀的负电荷，促进蛋白从凝胶转移到膜上。
3. 蛋白分子量>20kDa时，建议选择孔径0.45μm的PVDF膜。
4. 湿转时120V恒压转膜60-90分钟。为达到更好的转膜效果，可以根据预制胶上残留的预染marker及膜上的预染marker确定转膜效率，并对转膜条件进行适当调整。
5. 仅限于专业人员的科学研究用，不得用于临床医学诊断及其他非合理用途。不得存放于普通住宅内。
6. 为了您的安全和健康，请穿戴好个人防护装备和实验服装进行操作。
Aladdin UltraBio™ Tris-Acetate Precast PAGE Gel for High Molecular Weight Protein are safe, fast and high-performance precast gels, widely used for the separation and detection of high molecular weight (HMW) proteins.
Product Features:
⁕ Manufactured with automated gel casting technology to ensure high stability and reproducibility.
⁕ Effectively separates high molecular weight proteins ranging from 40 kDa to 500 kDa.
⁕ Equipped with glass plates to minimize non-specific protein adsorption, delivering sharper and clearer protein bands.
⁕ Short electrophoresis duration: complete run in 60 minutes at 150 V.
⁕ Easy cassette opening: gently score one side of the cassette with a blade for effortless separation.
⁕ Compatible with major mini electrophoresis systems including Bio-Rad, Invitrogen, Tanon, Junyi-Dongfang, Beijing Liuyi and others.
⁕ Available in homogeneous gels and gradient gels (7%, 3–8%). Customization for special concentrations is also provided.
Basic Information:
Gel Plate Dimensions: Width × Height × Thickness = 100 × 84 × 4.6 mm
Number of Wells: 10 wells, 15 wells
Gel Dimensions: Width × Height × Thickness = 81 × 74 × 1.5 mm
Gel Thickness: 1.5 mm
Maximum Sample Loading: 60 μL, 30 μL
Acr‑Bis Ratio: 37.5 : 1
Stacking Gel: 4%, 1.5 cm
Package: 10 gels per box
Shipping and Storage:
1. The product can be shipped at room temperature. If stored at room temperature, keep in a cool place, avoid extreme temperature fluctuations and direct sunlight.
2. Store at 2–8 °C for a shelf life of 2 months.
3. Do not store below 0 °C. Gels will freeze below 0 °C, forming bubbles and cracks, which will render the gels unusable.
Precast Gel Selection Guide:
T753564
| Concentration
| Wells
| Maximum Sample Loading Volume
| Running Buffer
| Transfer Buffer
| Separation Range
| Recommended Voltage
|
T753564-A1-10EA
| 7%
| 10
| 60 μl
| Tris-乙酸
| Tris-乙酸
| 35-300 kD
| 150 V
|
T753564-B1-10EA
| 7%
| 15
| 30 μl
| Tris-乙酸
| Tris-乙酸
| 35-300 kD
| 150 V
|
T753564-A2-10EA
| 3-8%
| 10
| 60 μl
| Tris-乙酸
| Tris-乙酸
| 45-400 kD
| 150 V
|
T753564-B2-10EA
| 3-8%
| 15
| 30 μl
| Tris-乙酸
| Tris-乙酸
| 45-400 kD
| 150 V
|
Instructions for Use:
1. Remove the UltraBio™ Precast Gel for High Molecular Weight Proteins (Tris-Acetate) from the package, clamp it into the electrophoresis tank, and assemble the electrophoresis unit.
2. Add 1× Tris-Acetate-SDS Running Buffer (T767965) to the tank. Fill the inner chamber completely; fill the outer chamber to at least 1/3 height, but not above the gel plates. Then gently pull out the comb slowly.
3. Gently pipette up and down in the wells to remove residual gel debris.
4. Sample loading: Mix protein sample 1:1 with 2× High Molecular Weight Protein Loading Buffer (S750310) thoroughly, then heat at 100 °C for 5–10 min. Avoid piercing the gel or inserting the tip too deeply, which may deform the gel and cause leakage during electrophoresis.
5. Connect the power supply and run at constant voltage 150 V. Stop electrophoresis when the bromophenol blue dye front reaches the bottom of the gel or the desired position.
6. After electrophoresis, take out the gel cassette. Use a blade to cut along the seam between the two glass plates to open the cassette. To prevent gel tearing or sticking, carefully slide the blade between the gel and glass strips. Caution: Be careful with the blade.
Precast Gel Separation Profile:
Mobility Profile Electrophoretogram
Gel Disassembly:
1. First, make a simple cut along the side gel (or remove excess gel-sealing material from the sides of the glass plate first);
2. Use a knife to cut through the gel-sealing material along the gap between the glass plate and the glass strip at the side gel (at the arrow), then gently open the glass plate;
3. When removing the gel, make a cut along the glass strips between the gel and the glass strips on both sides to prevent the gel from sticking and breaking during removal.
Electrophoresis Tanks Compatible with UltraBio™ Gel Mini Precast Gels
UltraBio™ Gel series precast gels are compatible with most mini SDS-PAGE electrophoresis tanks, including:
a. Bio-Rad Mini-PROTEAN (II/3/Tetra System)
b. Hoefer Mighty Small (SE250/SE260/SE280)
c. Life Technology Novex Mini-Cell (use with Aladdin custom cassette shield 01)
d. Life Mini Gel Tank (use with Aladdin custom cassette shield 02)
e. Beijing Liuyi DYCZ-25E, DYCZ-24K, DYCZ-24KS, DYCZ-24KF
f. Junyi-Dongfang JY-SCZ2+
g. Tanon VE180
or other electrophoresis tanks with a gel plate width of 10 cm.
Application in Life Electrophoresis Tanks: If required, please inform us when ordering this product
Place Baffle No. 01 on the outer side of the gel Place Baffle No. 02 on the inner side of the gel
Application in Bio-Rad Electrophoresis Tanks:
The top of the U-shaped sealing strip in the Bio-Rad Mini-PROTEAN series electrophoresis tanks has a raised structure, while the short glass plate of Aladdin’s UltraBio™ series precast gels has a concave structure—resulting in a flat surface at this location. Before electrophoresis, the sealing strip with the raised structure must be removed and reinstalled in reverse, with the smooth side facing outward, to prevent liquid leakage (as shown in the figure below).
a. Pull out the U-shaped sealing strip (the green part in the figure) from the Bio-Rad electrophoresis tank. Note that the two ends of the sealing strip have protrusions at this point: the side with protrusions is the front side, and the side without protrusions is the back side.
b. Rotate the sealing strip 180 degrees (with the front side facing inward and the back side facing outward) and reinstall it in the electrophoresis tank. Ensure the perimeter of the sealing ring is pressed firmly to prevent liquid leakage.
c. Place the precast gel and proceed with normal electrophoresis operations.
Precautions:
1. Reuse of electrophoresis buffer is not recommended. After electrophoresis, ionic strength and buffering capacity change, which cannot guarantee consistent electrophoresis performance. During transfer, increasing methanol concentration in transfer buffer improves transfer efficiency of low-molecular-weight proteins. Recommended methanol concentration: 20%–30%.
2. Methanol may cause precipitation of high-molecular-weight proteins. To avoid this, reduce methanol concentration in transfer buffer to 5%. To further prevent protein precipitation, add SDS to a final concentration of 0.1%. SDS provides uniform negative charge to proteins and facilitates their transfer from gel to membrane.
3. For proteins > 20 kDa, PVDF membrane with 0.45 μm pore size is recommended.
4. For wet transfer: run at 120 V constant voltage for 60–90 minutes. For better transfer efficiency, adjust conditions appropriately based on the remaining prestained marker in the precast gel and the marker transferred onto the membrane.
5. For research use only by professional personnel. Not for clinical diagnosis or other improper uses. Do not store in ordinary residential premises.
6. For your safety and health, wear personal protective equipment and laboratory clothing during operation.
ZJ26F0434667
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