计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
TMB是辣根过氧化物酶的底物。其可形成可溶性蓝色终产物,并可在370nm或655nm下读数。可以用0.5M H2SO4停止TMB反应(产生黄色溶液),并在450nm处读取数值。推荐用于ELISA(微孔)分析,不建议用于膜应用。通过将测量的底物吸收值超过吸收最大值,3,3′,5,5′-四甲基联苯胺已在研究中用于证明,任何免疫酶测定的测量范围都可以被显著延长,而又不损害测定特征。3,3′,5,5′-四甲基联苯胺也已用于研究中,以了解具有过氧化物酶样活性的CuS凹面上层结构的形成。
TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.