Tris EDTA缓冲液在分子生物学中常用于很多生物分子沉淀物(包括DNA和RNA)的重悬和保存。其中,Tris-HCl是常用缓冲液,EDTA是金属螯合剂。TE缓冲液可溶解核酸,同时保护其免受降解。可用于核酸分离,比如核酸凝胶电泳、Northern或Southern印迹杂交前的准备。
Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.
Application:
TE buffer has been used for:
isolate, resuspend, or store DNA and RNA for PCR or qPCR
gel electrophoresis, including Northern and Southern blotting
bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
antigen retrieval for immunohistochemistry
规格
规格或纯度
BioReagent, 100X concentrate for Northern and Southern blotting