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Mouse brain tissue lysate - total protein

Mouse brain tissue lysate - total protein

货号: tl155918

有货

应用

WB

规格

库存

价格

数量

200μg

tl155918-200μg

期货

¥3,655.00

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Mouse brain tissue lysate - total protein

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概述规格储存与运输图片技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器常见问题

🧪

为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-80℃储存。特低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE and WB analysis. The concentration was determined using the BCA assay process.

规格

产品名称

Mouse brain tissue lysate - total protein

规格或纯度

200μg

分子类型

细胞或组织提取物

产品线

生物产品

储存与运输

应用

WB

物理形态

冻干(Lyophilized)

储存缓冲液

Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).

复溶

Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

储存条件

-80℃储存

运输条件

特低温运输

稳定性与储存

Shipped on Dry Ice. Lyophilizate is recommended stored at -80°C. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20°C for short-term storage or -80°C for long-term storage.

图片

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: CAMK2B Mouse mAb (Ab092658) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 59 kDa
Exposure time: 49.2 s

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: ZnT-3 Antibody (Ab135449) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 6.6 s

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant PPP1CA/B Antibody (Ab122819) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38, 37 kDa
Observed band size: 35 kDa
Exposure time: 36.5 s

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Quinone oxidoreductase Antibody (Ab124077) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 17.7 s

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant Haptoglobin Antibody (Ab008379) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 39 kDa
Exposure time: 23.5 s

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: MSC Antibody (Ab116083) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 28 kDa
Exposure time: 1.8 s

Western Blot: Mouse brain tissue lysate - total protein (tl155918)

Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant Otx1 Antibody (Ab119495) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 100.0 s

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

常见问题

本产品应如何储存？

请于?80 °C条件下保存。需超低温保存以维持规定的有效期。

本产品如何运输？

本产品采用干冰配冷袋冷冻运输。收货后请立即拆包，并按上述储存条件保存；处理干冰时请戴隔热手套并保持通风。

如何获取批次专用COA？

每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。

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