Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE and WB analysis. The concentration was determined using the BCA assay process.
规格
产品名称
Mouse heart tissue lysate - total protein
规格或纯度
200μg
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Mouse heart tissue lysate - total protein (tl155919) - Western Blot All lanes: MYL4 Mouse mAb (Ab116764) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 26 kDa Exposure time: 14.5 s
危险品化学品经营许可证（带存储）