Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE and WB analysis. The concentration was determined using the BCA assay process.
规格
产品名称
Mouse liver tissue lysate - total protein
规格或纯度
200μg
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
应用
WB
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80°C. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20°C for short-term storage or -80°C for long-term storage.
图片
Mouse liver tissue lysate - total protein (tl155920) - Western Blot All lanes: PINCH-2 Mouse mAb (Ab121906) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 36 kDa Exposure time: 66.5 s
Mouse liver tissue lysate - total protein (tl155920) - Western Blot All lanes: Recombinant MSP/MST1 Antibody (Ab116146) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 79 kDa Exposure time: 100.0 s
Mouse liver tissue lysate - total protein (tl155920) - Western Blot All lanes: Recombinant CYP2D6 Antibody (Ab098589) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 56 kDa Observed band size: 48 kDa Exposure time: 3.1 s
危险品化学品经营许可证（带存储）