The Rat heart tissue lysate - total protein was prepared using a standard tissue lysate protocol. The concentration was determined using the BCA assay process.
规格
产品名称
Rat heart tissue lysate - total protein
别名
大鼠heart组织裂解物- total蛋白
规格或纯度
无载体, 无叠氮钠, 无支原体
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄).
复溶
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
图片
Rat heart tissue lysate - total protein (tl219055) - Western Blot All lanes: MYL4 Mouse mAb (Ab116764) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 26 kDa Exposure time: 14.5 s
危险品化学品经营许可证（带存储）