脲酶来源于刀豆

CAS: 9002-13-5 货号: U128713 EC号: 232-656-0

45篇引用文献 COA

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级别和纯度: EnzymoPure™ ? ≥45 units/mg dry weight

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数量

50mg

U128713-50mg

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¥441.90

250mg

U128713-250mg

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¥1,699.90

U128713-1g

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¥5,999.90

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为什么选择此级别

EnzymoPure™ 级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

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SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 45 篇同行评审文献引用。

概述

Jespersen (1975) reports that ammonium carbamate is produced in citrate and Tris buffer.
Urease occurs in many bacteria, several species of yeast and a number of higher plants. Varner (1960) has reviewed it. Two of the best sources are: Jack beans (Canavlia ensiformis) from which it has been crystallized and thoroughly studied, and Bacillus pasteurii.
The enzyme is important in assaying for urea. See Guilbault and Montalvo (1970). Its immobilization has been reported: et al. (1974), James and Pring (1975), Messing (1974), Nakamoto et al. (1975), Sundaram (1973) and Tran-Minh and Broun (1975).
Characteristics of Urease from Jack Bean:
Km: 1.3mM in Tris⋅HCl (Cesareo and Langton, 1992).
Specificity
Urease is specific for urea and hydroxyurea (Fishbein and Carbone 1965). See also Sundaram and Laidler (1970).
Composition
Monomeric (a)urease can polymerize to form six unit polymers of about three million daltons. (Fishbein et al. 1970; Fishbein and Nagarajan 1972a). Andrews and Reithel (1970) report on the sulfhydryl groups. Contaxis and Reithel (1971) indicate the molecule can be split in half with no loss of activity. See also Contaxis and Reithel (1972), Fishbein and Nagarajan (1972b), Lynn (1970), and Bailey and Boulter (1969).
Optimal pH：7.4
Stabilizers
EDTA in concentrations of 1 X 10-3 M. 50% glycerol solutions protect urease crystalline suspension for several months at 4°C.
Inhibitors
Heavy metals. NH4+ ions formed. See also Fishbein and Carbone (1965). Sodium and potassium ions are inhibitors (Cesareo and Langton, 1992).
Urease Assay
Method
Worthington has adopted an assay method where the hydrolysis of urea is measured by coupling ammonia production to a glutamate dehydrogenase reaction.
formulas
One unit results in the oxidation of one micromole of NADH per minute at 25°C and pH 7.6 under the specified conditions. In addition to increased sensitivity, the assay method possesses the advantage that it can be manipulated to permit quantitation of urea.
Reagents
0.1 M Potassium phosphate buffer, pH 7.6
0.023 M Adenosine-5'-diphosphate (ADP) in phosphate buffer
0.0072 M NADH in phosphate buffer
0.026 M a-Ketoglutarate in phosphate buffer
1.8 M Urea in phosphate buffer
Glutamate Dehydrogenase: Dilute to approximately 500 units/ml in 50% glycerol or phosphate buffer. Store cold during use.
Enzyme
Dissolve enzyme at one mg/ml in 0.1 M phosphate buffer, pH 7.6. Immediately prior to use, dilute further in buffer to obtain a rate of 0.02-0.04 ΔA/minute.
Procedure
Adjust spectrophotometer to 340 nm and 25°C. Pipette into each cuvette as follows:

0.10 M Phosphate buffer, pH 7.6 2.4 ml

0.023 M ADP 0.1 ml

0.0072 M NADH 0.1 ml

0.026 M α-Ketoglutarate 0.1 ml

1.8 M Urea 0.1 ml

GLDH (500 units/ml) 0.1 ml

Incubate in spectrophotometer at 25°C for 5-10 minutes to achieve temperature equilibration and establish blank rate, if any. A slight change in absorbance may be observed due to trace ammonia in reagents. Upon obtaining a zero change in absorbance, add 0.1 ml appropriately diluted enzyme. Record decrease in A340 for 8-10 minutes. Determine ΔA340/minute from the linear portion of the curve. A slight lag may occur.

Calculation

规格

产品名称

脲酶来源于刀豆

别名

尿素酶

英文别名

Urea amidohydrolase

规格或纯度

EnzymoPure™, ≥45 units/mg dry weight

生化机理

尿素酶催化尿素水解为二氧化碳和氨。尿素酶参与氮代谢和尿素降解。鱼腥草中的尿素酶每个亚基结合 2 个镍离子。

CAS编号和信息

9002-13-5

酶学委员会编号

3.5.1.5

分子类型

酶

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥45 units/mg dry weight

储存条件

-20°C储存

运输条件

超低温运输

单位定义

One Unit oxidizes one micromole of NADH per minute at 25°C, pH 7.6. The hydrolysis of urea is measured by coupling ammonia production to a glutamate dehydrogenase reaction.

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批号(Lot Number)	证书类型	货号
C2511609	分析证书	U128713
C2511610	分析证书	U128713
C2511619	分析证书	U128713
K2526047	分析证书	U128713
B2510038	分析证书	U128713
J2409536	分析证书	U128713
J2409537	分析证书	U128713
J2409538	分析证书	U128713
G2317037	分析证书	U128713
G2317079	分析证书	U128713
G2317096	分析证书	U128713
G2426152	分析证书	U128713
B2318515	分析证书	U128713
H2209752	分析证书	U128713

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技术文档和文章

脲酶反应体系、尿素转化与氨氮定量分析

目录：技术文章

探索主题:

脲酶活性尿素代谢氨氮尿素氮靛酚蓝法 Berthelot反应二乙酰一肟法土壤脲酶

Urease Reaction Systems, Urea Conversion, and Ammonia Nitrogen Quantitative Analysis

目录：技术文章

探索主题:

Urease activity Urea metabolism Ammonia nitroge Urea nitrogen Indophenol blue method Berthelot reaction Diacetyl monoxime method Soil urease

尿素检测方法比较：脲酶法与二乙酰一肟法的反应机制、干扰因素和适用场景

目录：技术文章

探索主题:

比色法二乙酰一肟法尿素检测尿素氮检测 BUN 脲酶法尿素酶 DAM法血清尿素尿液尿素尿素标准品 GLDH偶联法吲哚酚显色法氨干扰临床生化检测

Comparison of Urea Detection Methods: Reaction Mechanisms, Interference Factors, and Application Scenarios of the Urease Method and Diacetyl Monoxime Method

目录：技术文章

探索主题:

Diacetyl monoxime method BUN DAM method GLDH-coupled method Urea Detection Urea Nitrogen Detection Urease Method Urease Serum Urea Urinary Urea Urea Standard Indophenol Colorimetric Method Ammonia Interference Colorimetric Method Clinical Biochemical Detection

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