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避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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蛋白质远离等电点，易促使蛋白形成不溶性沉淀，磺基水杨酸为微生物碱，在酸性条件下其阴离子可与带正电荷的蛋白质结合成不溶性蛋白盐而沉淀。该产品属于定性检测试剂盒，灵敏度可达0.05～0.1g/L，多用于人或动物尿液蛋白质的检查。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
| U1506746 | Component | 100T | Storage |
| U1506746A | 磺基水杨酸溶液 | 10 mL | RT. Store in the dark. |
| U1506746B | 酸化液 | 10 mL | RT. |
实验流程
1、取3～5ml新鲜尿液转移入小试管。
2、滴加磺基水杨酸溶液1～2滴，形成界面。
4、结果判断
| 结果判定 | 现象描述 | 预估蛋白浓度 |
| 阴性 (-) | 不显浑浊，清晰透明 | < 0.05 g/L |
| 可疑 (±) | 轻微浑浊，隐约可见 | 0.05 - 0.2 g/L |
| (+) | 明显白色浑浊，无颗粒 | ~ 0.3 g/L |
| (2+) | 稀薄乳样浑浊，出现颗粒 | ~ 1 g/L |
| (3+) | 乳浊，有絮片状沉淀 | ~ 3 g/L |
| (4+) | 絮片状沉淀，凝固成块 | > 5 g/L |
注意事项
1、如待测尿液浑浊，应先离心或过滤。
2、强碱性尿易出现假阳性。
3、本法灵敏度为0.05～0.1g/L。
4、强碱性尿液易出现假阴性，应加滴加数滴酸化溶液后再作检测。
5、有机造影剂、大剂量青霉素或尿液中含高浓度尿酸或尿酸盐，可致假阳性。
6、尿液含有高浓度尿素或尿酸盐时，可呈假阳性。
7、试剂开封后请尽快使用，以防影响后续实验效果。
When proteins are far from their isoelectric point, they tend to form insoluble precipitates. Sulfosalicylic acid is a biological alkaloid reagent. Under acidic conditions, its anions can combine with positively charged proteins to form insoluble protein salts, resulting in precipitation. This product is a qualitative detection kit with a sensitivity of 0.05-0.1 g/L, primarily used for detecting proteins in human or animal urine. This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes
| U1506746 | Component | 100T | Storage |
| U1506746A | Sulfosalicylic Acid Solution | 10 mL | RT. Store in the dark. |
| U1506746B | Acidification Solution | 10 mL | RT. |
Experimental Procedure
Transfer 3-5 ml of fresh urine into a small test tube.
Add 1-2 drops of Sulfosalicylic Acid Solution, allowing it to form a layer/interface.
Observe immediately. If turbidity appears, it indicates the presence of protein in the urine. The depth of turbidity corresponds to the approximate concentration.
Result Interpretation
| Result Interpretation | Phenomenon Description | Estimated Protein Concentration |
| Negative (-) | No turbidity, clear and transparent | < 0.05 g/L |
| Suspicious (±) | Slight turbidity, faintly visible | 0.05 - 0.2 g/L |
| (+) | Distinct white turbidity, no granules | ~ 0.3 g/L |
| (2+) | Thin, milky turbidity with appearing granules | ~ 1 g/L |
| (3+) | Milky and opaque, with flocculent precipitates | ~ 3 g/L |
| (4+) | Flocculent precipitates, coagulated into clumps | > 5 g/L |
Precautions
If the urine sample is turbid, it should be centrifuged or filtered first.
Highly alkaline urine may cause false-positive results.
The sensitivity of this method is 0.05-0.1 g/L.
Highly alkaline urine may also lead to false-negative results. Add a few drops of the Acidification Solution and retest.
False-positive results can be caused by organic contrast agents, large doses of penicillin, or high concentrations of uric acid/urates in the urine.
High concentrations of urea or urates in the urine may cause false-positive results.
Use reagents promptly after opening to prevent potential effects on subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131312 |
分析证书 | U1506746 |
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