计算溶液所需的质量、体积或浓度。
尿含铁血黄素定性检测试剂盒（Rous法）
有货
为什么选择此级别
BioReagent,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
含铁血黄素（Hemosiderin）是一种血红蛋白源性色素，为金黄色或棕黄色颗粒，因其含铁且为金黄色，故称为含铁血黄素，是不稳定的铁蛋白聚合体，为含铁质的棕色色素。当尿中存在含铁血黄素时，在酸性条件下亚铁氰化钾溶液使三价铁离子从蛋白质中被稀盐酸分离出来，三价铁与亚铁氰化钾反应，生成一种不溶解的蓝色化合物即三价铁的亚铁氰化物称为普鲁士蓝反应。
尿含铁血黄素定性检测试剂盒（Rous法）是特异地检测尿液中含铁血黄素的试剂盒，定性好、不易产生沉淀、应用范围广，但不适用于组织或细胞的染色。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤
1、取新鲜尿液5～10ml，2000g离心5min，弃上清。
2、向沉渣（即沉淀）中加入Perls Solution A、Perls Solution B各1～2ml，充分均匀，室温静置10min。
3、2000g离心5min，弃上清，取沉淀物涂片，加盖盖玻片后高倍镜下观察，必要时可用油镜。
4、结果判断：分散或成堆蓝色闪光颗粒即为阳性，如果在细胞内更可信。
注意事项
1、待测尿液应新鲜。
2、所有试剂、玻片、器皿均应防止铁剂污染，否则易出现假阳性。
3、一般应做阴性对照，Perls Solution A、B混合呈蓝色，说明试剂已经污染，应弃用。
4、试剂开封后请尽快使用，以防影响后续实验效果。
Hemosiderin is a hemoglobin‑derived pigment that appears as golden‑yellow or brownish‑yellow granules. It is called hemosiderin because it contains iron and exhibits a golden‑yellow color. It is an unstable polymer of ferritin and presents as an iron‑containing brown pigment. When hemosiderin is present in urine, under acidic conditions, potassium ferrocyanide solution releases ferric ions from proteins with the aid of dilute hydrochloric acid. The ferric ions then react with potassium ferrocyanide to form an insoluble blue compound known as ferric ferrocyanide, a reaction termed the Prussian blue reaction.
The Urine Hemosiderin Qualitative Detection Kit (Rous Method) is specifically designed for the detection of hemosiderin in urine. It offers good specificity, minimal precipitation, and broad applicability, but is not suitable for staining tissues or cells. This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Procedure
1. Take 5–10 mL of fresh urine and centrifuge at 2000 g for 5 minutes. Discard the supernatant.
2. Add 1–2 mL each of Perls Solution A and Perls Solution B to the sediment (pellet). Mix thoroughly and let stand at room temperature for 10 minutes.
3. Centrifuge at 2000 g for 5 minutes. Discard the supernatant, smear the sediment onto a slide, cover with a coverslip, and observe under high‑power magnification. Use oil immersion if necessary.
4. Interpretation of Results:
The presence of scattered or clustered blue‑glittering granules indicates a positive result. Granules located inside cells are considered more reliable.
Notes
1. Urine samples should be fresh.
2. All reagents, slides, and utensils must be free from iron contamination to avoid false‑positive results.
3. A negative control should generally be performed. If the mixture of Perls Solution A and B appears blue, it indicates reagent contamination and should be discarded.
4. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
尿含铁血黄素检测试剂盒 | 尿含铁血黄素检测试剂盒
英文别名
Urinary Hemosiderin Qualitative Test Kit (Rous Method) | Urinary Hemosiderin Qualitative Test Kit (Rous Method)
规格或纯度
BioReagent, 用于显微镜
英文名称
Urine Hemosiderin Qualitative Assay Kit (Rous Method)
样品类型
尿液
储存与运输
储存条件
室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (12 months).
组分与储存
| U1507812 | Component | 50T | Storage |
| U1507812A | Perls Solution A | 100 mL | RT. |
| U1507812B | Perls Solution B | 100 mL | RT. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131502 |
分析证书 | U1507812 |
技术文档和文章
溶液计算器
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