BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿胆原又称为尿胆素原，是老旧的红细胞在肝脏或脾脏遭到破坏，血红素会变成间接胆红素，在肠内被细菌所分解代谢而变成尿胆原。
检测原理：在酸性条件下，Ehrlich试剂与尿胆原反应，生成红色化合物，该试剂盒灵敏度高，主要用于定性检测人、动物尿液中的尿胆原。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
自备仪器和试剂
操作步骤（仅供参考）
1、（备选）如果尿液中含有胆红素，应去除胆红素，即取新鲜尿液4mL置于试管，加入氯化钡溶液约1mL，混匀，3000g离心5min，弃沉淀，留取上清液备用。
2、取新鲜无胆红素尿液2mL，加入0.2mL Ehrlich试剂，静置10min，观察结果。
结果判断
观察方法：在白色背景下，从管口直视管底。
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注意事项
1、尿液必须新鲜，久置后尿胆原氧化成尿胆素，呈假阴性。
3、如果抗生素抑制了肠道菌群，可使尿胆原减少或者缺乏。
4、试剂开封后请尽快使用，以防影响后续实验效果。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Urobilinogen, also known as urochrome, is formed when aged red blood cells are broken down in the liver or spleen. Hemoglobin is converted into indirect bilirubin, which is then metabolized by intestinal bacteria into urobilinogen.
Detection Principle
Under acidic conditions, Ehrlich's reagent reacts with urobilinogen to produce a red-colored compound. This kit is highly sensitive and is primarily used for the qualitative detection of urobilinogen in human or animal urine. This kit is for research use only and is not suitable for clinical diagnosis or other purposes.
Reagents, consumables and Equipments not provided
Operating Steps (For Reference Only)
1. (Optional) If the urine sample contains bilirubin, it should be removed. Take 4 mL of fresh urine into a test tube, add approximately 1 mL of Barium Chloride Solution, mix well, centrifuge at 3000 g for 5 minutes, discard the precipitate, and keep the supernatant for use.
2. Take 2 mL of fresh, bilirubin‑free urine, add 0.2 mL of Ehrlich's Reagent, let stand for 10 minutes, and observe the result.
Result Interpretation
Observation method: Observe from the top of the tube against a white background, looking directly to the bottom.
| Result | Observation |
| Negative | No red color appears. |
| Positive (+) | Appears faint red. |
| Positive (2+) | Appears cherry red. |
| Positive (3+) | Immediately appears deep red. |
Note: Normal urine should give a positive (+) result.
Precautions
1. Urine must be fresh. Urobilinogen oxidizes to urobilin upon prolonged standing, leading to false‑negative results.
2. If the urine contains interfering substances such as porphobilinogen, add 2 mL of chloroform after adding Ehrlich's Reagent. Shake well and let stand. Urobilinogen will transfer to the chloroform layer, confirming a positive result.
3. If antibiotics have suppressed the intestinal flora, urobilinogen may be reduced or absent.
4. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
| U1509467 | Component | 100T | Storage |
| U1509467A | 氯化钡溶液 | 50 mL | RT. |
| U1509467B | Enrlich试剂 | 20 mL | RT. Store in the dark. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333467 |
分析证书 | U1509467 |
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