计算溶液所需的质量、体积或浓度。
概述
| 货号 | 粒径 | 吸收波长 | PDI值 | OD值 |
| U1521586-01 | 20nm | λmax 515~519nm | ＜0.2 | 100 |
| U1521586-02 | 30nm | λmax 520~522nm | ＜0.2 | 100 |
| U1521586-03 | 40nm | λmax 523~526nm | ＜0.2 | 100 |
| U1521586-04 | 50nm | λmax 528~532nm | ＜0.2 | 100 |
| U1521586-05 | 60nm | λmax 534~539nm | ＜0.2 | 100 |
| U1521586-06 | 70nm | λmax 540~544nm | ＜0.2 | 100 |
| U1521586-07 | 80nm | λmax 545~548nm | ＜0.2 | 100 |
胶体金（Gold Colloid）是氯金酸在还原剂的作用下制成的胶体状态的金颗粒，因其表面带 有负电荷，可与蛋白等分子表面的正电荷相互作用，使蛋白等牢固结合在胶体金表面。因此，胶体金作为示踪物，广泛应用于免疫诊断产品中。传统方法制备的胶体金均一性差，浓度低（0.01%-0.04%）。 阿拉丁的胶体金均一性好，呈单分散（粒径 CV ＜8%），浓度高（1%），且具有标记效率高、节省标记原料和能耗、操作简便等特点。
产品优势
1、节省活材：高浓度胶体金标记效率高，节省50%以上活材；
2、缩短供货周期：高浓度作业，减少离心时间，减少大型离心设备投入，缩短供货周期；
3、单分散性能优：PDI低于0.2，减小批间差。
产品浓度
100±5OD（最大紫外吸收波长处）
使用方法
I 最佳标记条件的确认：
1. 用不同 pH 的缓冲液，将超高浓度胶体金稀释至 40OD（如：40μL 超高浓度胶体金+60μL 缓冲液）。
2. 分别加入相同浓度的标记蛋白（抗原或抗体，推荐标记浓度 50μg/mL~120μg/mL），室温震荡反应 15min~2h。
3. 反应结束后取少量标记后的浓缩金溶液，用纯化水将其稀释至 1 OD（如：25μL 标记后的超高浓度胶体金+975μL 纯化水）。
4. 向每毫升 1OD 的标记后的胶体金溶液中，加入 100μL 的 10% NaCl，震荡混匀后，观察 颜色变化情况。5 分钟后，颜色变化最小或聚沉最少的条件，即为该标记蛋白的最佳标记pH。
5. 如以上 pH 缓冲液的条件均变色或聚沉，可在标记缓冲液中加入 0.04%NaCl 或提高标记浓度，再进行上述最适 pH 的确认。
*注：建议使用 10mM 的磷酸盐缓冲液、硼酸-硼砂缓冲液、碳酸盐缓冲液。
II 超高浓度胶体金的标记：
用筛选出的最佳标记条件进行标记。
III 封闭：
加入一定量的胶体金封闭剂，震荡反应 20 分钟。
IV 纯化及复溶：
离心→弃上清→用胶体金复溶液将沉淀复溶到相应浓度即可。
注意事项
a) 贮存：本产品可在室温条件下运输。收到后建议 4℃~8℃保存，避免放置在冷藏室出风口处
和内壁处，严禁冷冻保存。长期放置出现的分层、瓶内壁因水分蒸发造成的金斑点属于正常现
象，一般不影响使用。
b) 取用或分装时，请使用干净的容器，以免污染造成胶体金聚集，建议使用 PC 透明材质。
c) 开瓶后请在 2 个月内使用完毕。
d) 胶体金标记过程中所用的缓冲液，建议使用 BND 或 NaN3 防腐剂，避免使用 ProClin 系列。
e) 长期储存后，胶体金溶液 pH 值可能会发生变化，建议使用之前进行标记条件再优化。
免责声明
a) 因储存或使用不当造成的贴壁、聚沉、死金或者胶体金非正常态的情况，需自行承担责任。
b) 产品检验合格后再使用，如因未进行产品检验而直接使用造成的后果，需自行承担责任。
Gold Colloid is a colloidal gold particle produced from chloroauric acid under the influence of a reducing agent. Due to its negatively charged surface, it can interact with positively charged molecules such as proteins, allowing them to bind firmly to the surface of the colloidal gold. Therefore, gold colloid, as a tracer, is widely used in immunodiagnostic products. Gold colloids prepared by traditional methods exhibit poor uniformity and low concentration (0.01%-0.04%). Aladdin's gold colloids demonstrate excellent uniformity, exhibiting monodispersity (particle size CV < 8%) and high concentration (1%), and they also feature high labeling efficiency, saving labeling materials and energy consumption, and simple operation.
Product advantages
1、Saving active materials: High-concentration colloidal gold has high labeling efficiency, saving more than 50% of active materials;
2、Shortening supply cycle: High-concentration operation reduces centrifugation time, reduces the investment in large centrifuge equipment, and shortens the supply cycle;
3、Excellent monodispersity: PDI is below 0.2, reducing batch-to-batch variability.
Product Concentration
100±5 OD (at the maximum UV absorption wavelength)
Instructions for Use
I. Determination of Optimal Labeling Conditions
1、Dilute ultra-high concentration colloidal gold to 40 OD using buffers of different pH values (e.g., 40 μL ultra-high concentration colloidal gold + 60 μL buffer).
2、Add labeling protein (antigen or antibody) at the same concentration to each sample; the recommended labeling concentration is 50 μg/mL–120 μg/mL. React with shaking at room temperature for 15 min to 2 h.
3、After the reaction, collect a small volume of the labeled concentrated gold solution and dilute it to 1 OD with purified water (e.g., 25 μL labeled ultra-high concentration colloidal gold + 975 μL purified water).
4、Add 100 μL of 10% NaCl to each milliliter of the 1 OD labeled colloidal gold solution. Mix by shaking and observe the color change. The condition showing the least color change or minimal aggregation after 5 minutes is the optimal labeling pH for the labeling protein.
5、If all tested pH buffer conditions result in color change or aggregation, add 0.04% NaCl to the labeling buffer or increase the labeling concentration, then repeat the above optimal pH determination procedure.
*Note: 10 mM phosphate buffer, borate-borax buffer, or carbonate buffer is recommended.
II. Labeling of Ultra-High Concentration Colloidal Gold
Perform labeling using the optimal conditions determined in the screening step.
III. Blocking
Add an appropriate amount of colloidal gold blocking agent and react with shaking for 20 minutes.
IV. Purification and Resuspension
Centrifuge → Discard the supernatant → Resuspend the pellet with colloidal gold resuspension buffer to the target concentration.
Precautions
a). Storage: This product can be transported at room temperature. After receipt, storage at 4 °C–8 °C is recommended. Avoid placing it at air vents or inner walls of the refrigerator compartment. Freezing is strictly prohibited. Stratification after long-term storage and gold spots on the inner bottle wall caused by water evaporation are normal phenomena and generally do not affect usage.
b). When aliquoting or dispensing, use clean containers to prevent colloidal gold aggregation due to contamination. PC transparent material is recommended.
c). Use the product within 2 months after opening.
d). For buffers used in colloidal gold labeling, BND or NaN₃ preservatives are recommended; the ProClin series should be avoided.
e). The pH of the colloidal gold solution may change after long-term storage. Re-optimization of labeling conditions prior to use is recommended.
Disclaimer
a). Any adhesion, aggregation, dead gold, or abnormal state of colloidal gold caused by improper storage or use shall be the sole responsibility of the user.
b). The product must be inspected and verified as qualified before use. Any consequences arising from direct use without prior product inspection shall be the sole responsibility of the user.
规格
规格或纯度
100OD,PDI ≤0.2,用于体外诊断
英文名称
Ultra-high concentration Gold Colloid
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
未知
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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