BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿素(Urea)又称碳酰胺(carbamide)，是哺乳动物和某些鱼类体内蛋白质代谢分解的主要含氮终产物，也是目前含氮量最高的氮肥；尿素检测方法大致分为化学方法和酶学方法，后者被认为是间接方法，先经尿素酶分解尿素为铵离子，然后根据波氏反应检测铵离子的生成量。 该试剂盒检测原理是尿素酶水解尿素，产生氨和二氧化碳，铵离子与苯酚反应生成蓝色吲哚酚，吲哚酚的生成量与尿素含量呈正比，通过分光光度计测定560nm处吸光度，专门用于测定人体、动物的尿液尿素(旧称尿素氮，BUN)，也可用于测定血清、血浆等样品中尿素含量。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Urea, also known as carbamide, is the main nitrogen-containing end product of protein metabolism in mammals and certain fish species, and it is also the nitrogen fertilizer with the highest nitrogen content currently available. Urea detection methods can be broadly categorized into chemical methods and enzymatic methods. The latter is considered an indirect method, where urea is first broken down into ammonium ions by urease, and then the amount of ammonium ions generated is detected based on the Berthelot reaction. The detection principle of this kit is based on the hydrolysis of urea by urease, producing ammonia and carbon dioxide. The ammonium ions react with phenol to form blue indophenol. The amount of indophenol formed is proportional to the urea content. By measuring the absorbance at 560 nm with a spectrophotometer, this kit is specifically designed for determining urea content (formerly referred to as blood urea nitrogen, BUN) in human and animal urine, but can also be used to measure urea content in samples such as serum and plasma. This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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