尿素(Urea)又称碳酰胺(carbamide)，是哺乳动物和某些鱼类体内蛋白质代谢分解的主要含氮终产物，也是目前含氮量最高的氮肥；尿素检测方法大致分为化学方法和酶学方法，后者被认为是间接方法，先经尿素酶分解尿素为铵离子，然后根据波氏反应，检测铵离子的生成量。 该试剂盒检测原理是在酸性条件下加热一定时间，尿素与二乙酰缩合，生成红色二嗪(diazine)，该反应被称为Fearon反应，颜色深浅与尿素含量呈正比，测定540nm处吸光度，该试剂盒可用于检测人体、动物的血浆、血清、尿液等样品中尿素(旧称尿素氮，BUN)含量，尿液样品可直接检测，无需处理。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Urea, also known as carbamide, is the main nitrogen-containing end product of protein metabolism in mammals and certain fish species, and it is also the nitrogen fertilizer with the highest nitrogen content currently available. Urea detection methods can be broadly categorized into chemical methods and enzymatic methods. The latter is considered an indirect method, where urea is first broken down into ammonium ions by urease, and then the amount of ammonium ions generated is detected based on the Berthelot reaction. The detection principle of this kit is based on the condensation reaction between urea and diacetyl monoxime under acidic conditions and upon heating for a specific period, forming a red-colored compound called diazine. This reaction is known as the Fearon reaction. The intensity of the red color is proportional to the urea content. By measuring the absorbance at 540 nm, this kit can be used to determine the urea content (formerly referred to as blood urea nitrogen, BUN) in samples such as human and animal plasma, serum, and urine. Urine samples can be detected directly without any pretreatment. This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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