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UDPG焦磷酸化酶（UGP，EC 2.7.7.9）是碳水化合物代谢的关键酶之一，广泛分布于微生物、动植物及多种组织中。该酶催化葡萄糖的活化反应，将1-磷酸葡萄糖与UTP合成为UDP-葡萄糖（UDPG），为蔗糖、葡聚糖、纤维素、半纤维素、果胶质及糖蛋白等多种碳水化合物的合成提供葡萄糖基供体。本试剂盒基于UGP可逆催化反应生成1-磷酸葡萄糖的原理，在磷酸葡萄糖变位酶（PGM）和6-磷酸葡萄糖脱氢酶（G6PDH）的偶联作用下，将NADP⁺还原为NADPH。通过监测340 nm处NADPH特征吸收峰的增加速率，在底物饱和条件下，单位时间内OD₃₄₀的初始线性增幅（ΔOD₃₄₀/min） 与UGP酶活性呈正比，据此即可精准计算样品中UGP的酶活力大小。
UDPG pyrophosphorylase (UGP, EC 2.7.7.9) is a key enzyme in carbohydrate metabolism, widely distributed in microorganisms, animals, plants, and various tissues. This enzyme catalyzes the activation of glucose by condensing glucose-1-phosphate with UTP to form UDP-glucose (UDPG), which serves as a glucosyl donor for the synthesis of various carbohydrates, including sucrose, glucans, cellulose, hemicellulose, pectin, and glycoproteins. This kit is based on the principle that UGP catalyzes the reversible reaction to generate glucose-1-phosphate, which, through the coupled actions of phosphoglucomutase (PGM) and glucose-6-phosphate dehydrogenase (G6PDH), reduces NADP⁺ to NADPH. By monitoring the rate of increase in the characteristic absorbance peak of NADPH at 340 nm, under substrate-saturated conditions, the initial linear increase in OD₃₄₀ per unit time (ΔOD₃₄₀/min) is proportional to UGP activity. Accordingly, the UGP enzyme activity in the sample can be precisely calculated.
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