脲酶 (URH)

Name: 脲酶 (URH)
SKU: U754676
Availability: InStock

CAS: 9002-13-5 货号: U754676 EC号: 232-656-0

45篇引用文献 COA

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级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? 来源于刀豆; ≥100 U/mg enzyme powder; ≥150 U/mg protein

Accession #

P07374

生物活性

≥100 U/mg enzyme powder; ≥150 U/mg protein

规格

库存

价格

数量

10KU

U754676-10KU

现货

¥249.90

50KU

U754676-50KU

现货

¥849.90

250KU

U754676-250KU

现货

¥2,599.90

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 45 篇同行评审文献引用。

概述

脲酶存在于胞质溶胶中。它在酸性 pH 下具有活性，在 28℃ 下表达。脲酶结合镍，存在于古细菌、细菌、单细胞真核生物和植物中。矮刀豆脲酶是第一个被结晶的酶，同时也是第一个被发现的含镍酶。它是一种多亚基酶，由三种蛋白质形式的91 kDa亚基组成。

亚基分子量：约 90,770

由六个亚基组成，总分子量：约 544620

应用

来自刀豆的脲酶已被用于：对尿样加标以测定纯尿中尿素的水解速率它还被用于研究多卤代苯并萘醌和萘醌对巴斯德芽孢杆菌和洋刀豆的抑制作用。

生化/生理学作用

脲酶参与嘌呤代谢和尿素循环。它水解尿素以生产氨和二氧化碳。在洋刀豆（Jack Bean）中，脲酶促进L-刀豆氨酸转化为副刀豆氨酸

Urease is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.Subunit molecular weight: ~90,770

Composed of six subunits with total molecular weight: ~544,620

Application

Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.

Biochem/physiol Actions

Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.

规格

产品名称

脲酶 (URH)

别名

尿素酶

英文别名

Urea amidohydrolase | acid urease | Urea amidohydrolas

规格或纯度

生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,来源于刀豆; ≥100 U/mg enzyme powder; ≥150 U/mg protein

生化机理

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Is known to be highly toxic and lethal when given by intravenous route, producing convulsions and other signs of central nervous system intoxication associated with the high levels of ammonia formed in the blood of mice and rabbits. Is neurotoxic in mammals, when directly injected into hippocampus. It may induce seizures by acting at a neuronal network level, thereby disturbing electroencephalographic rhythms and causing metabolic alterations in key areas related to epileptogenesis and to neurogenic pulmonary edema. It increases calcium influx and neuronal firing rate in the hippocampus. Is able to insert itself into lipid bilayers, altering physicochemical properties of artificial membranes, and forming cation-selective ion channels. In vitro, has the ability to induce platelet aggregation, platelet granules secretion and release of ATP. In contrast to canatoxin, another urease from C.ensiformis, is not lethal to mice when intraperitoneally injected. Miscellaneous: Was isolated in 1926 by James B. Sumner. It was the first enzyme ever obtained in a crystalline form, demonstrating that enzymes could be proteins. Post-translational: Carboxylation allows a single lysine to coordinate two nickel ions.

生物活性

≥100 U/mg enzyme powder; ≥150 U/mg protein

种属

刀豆(Jack Bean)

来源

天然

CAS编号和信息

9002-13-5

酶学委员会编号

EC 3.5.1.5

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

来源于刀豆; ≥100 U/mg enzyme powder; ≥150 U/mg protein

复溶

Reconstitute in water to a concentration of 10 mg/ml.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

短期储存2-8℃（12个月）；长期储存-20℃（24个月）；复溶后建议分装，避免反复冻融。

单位定义

1 U Corresponds to the Amount of Enzyme which Hydrolyzes 1 µmol Urea per Minute at ph 8.0 and 25 deg C

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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🔬 规格说明书

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高级数据

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ26F0636950	分析证书	U754676
ZJ26F0636949	分析证书	U754676
ZJ26F0636948	分析证书	U754676
I2504666	分析证书	U754676

技术文档和文章

u754676 urease from canavalia ensiformis jack bean product manual.pdf

纯净提取，智慧溶剂：天然提取试剂实践手册

目录：阿拉丁试剂的规格、级别和纯度

探索主题:

级别天然提取试剂

Clean Extracts, Smart Solvents: The Natural Extraction Reagent Playbook

目录：阿拉丁试剂的规格、级别和纯度

探索主题:

grade Natural Extraction Reagents

脲酶反应体系、尿素转化与氨氮定量分析

目录：技术文章

探索主题:

脲酶活性尿素代谢氨氮尿素氮靛酚蓝法 Berthelot反应二乙酰一肟法土壤脲酶

Urease Reaction Systems, Urea Conversion, and Ammonia Nitrogen Quantitative Analysis

目录：技术文章

探索主题:

Urease activity Urea metabolism Ammonia nitroge Urea nitrogen Indophenol blue method Berthelot reaction Diacetyl monoxime method Soil urease

尿素检测方法比较：脲酶法与二乙酰一肟法的反应机制、干扰因素和适用场景

目录：技术文章

探索主题:

比色法二乙酰一肟法尿素检测尿素氮检测 BUN 脲酶法尿素酶 DAM法血清尿素尿液尿素尿素标准品 GLDH偶联法吲哚酚显色法氨干扰临床生化检测

Comparison of Urea Detection Methods: Reaction Mechanisms, Interference Factors, and Application Scenarios of the Urease Method and Diacetyl Monoxime Method

目录：技术文章

探索主题:

Diacetyl monoxime method BUN DAM method GLDH-coupled method Urea Detection Urea Nitrogen Detection Urease Method Urease Serum Urea Urinary Urea Urea Standard Indophenol Colorimetric Method Ammonia Interference Colorimetric Method Clinical Biochemical Detection

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