VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.
This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Specifications
Molecular Weight
105 kDa
Extinction Coefficient
0.78
Inhibiting or Eluting Sugar
GalNAc
Storage Instructions
2-8 ℃
Sugar Specificity
Terminal GalNAc and terminal LacdiNAc
Usage Summary
Although many buffers can be employed for reconstituting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl₂. For preserving solutions stored at 4 ℃, 0.08% sodium azide can be used.
Applications
Glycobiology
Conjugate
Unconjugated
规格
产品名称
野豌豆凝集素 (VVL)
英文别名
Lectin from Vicia Villosa | VVA
规格或纯度
BioReagent, 天然
种属
蓖麻(Ricinus Communis)
偶联
Unconjugated
来源
天然
预测分子量
105 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
复溶
Reconstitute in 10 mM HEPES buffered saline, 0.1 mM CaCl₂, pH 8.5. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.