病毒保存液主要用于组织、细胞病理学分析样本的保存,不仅包括冠状病毒、流感病毒、手足口病毒、麻疹风疹病毒,也包括其他类别的病毒和支原体、脲原体,在储存及运输过程中能够保持病毒核酸的完整性。
病毒保存液 (灭活型, 含胍盐, 红色) 含pH缓冲剂、胍盐、渗透压维持剂、抗生素、防腐剂、酚红等,将采集到的具有代表性的病毒保存于本产品后进行核酸RNA或DNA的提取,能保存细胞或病毒的完整性。将鼻、咽拭子置于病毒保存液后,其胍盐是常用的核酸酶活性抑制剂,可以保护核酸RNA免受RNA酶的降解,以及保护脱氧核糖核酸DNA免受DNA酶的降解;联合使用多种抗生素和防腐剂使该产品具有较强的抗细菌和抗真菌作用;酚红可用来监测和指示溶液的pH状态,在一些特定的实验中,如果存在对酚红敏感的应用(例如某些细胞培养或分子生物学实验),可能需要考虑使用不含酚红的保存液或培养基。该产品仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
1、根据不同目的,用采样拭子或其他工具在相应的部位进行病毒采样。
2、置于病毒保存液中,可2~ 8°C短时间 (不超过48小时) 保存和运输。
注意事项:
1、处理鼻涕液或痰液标本时,应注意充分稀释和混匀。
2、采集标本必须保证新鲜,以免细胞自溶。
3、本试剂对眼睛和皮肤有一定的刺激性,若不慎溅入眼睛即用清水冲洗,使用时应当保持通风。
4、本产品仅用于科研领域,不适用于临床诊断或其他用途。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Virus preservation solution is mainly used for the preservation of samples for tissue and cytopathological analysis. It is applicable not only to coronaviruses, influenza viruses, hand-foot-and-mouth disease viruses, measles and rubella viruses, but also to other types of viruses, mycoplasmas and ureaplasmas. It can maintain the integrity of viral nucleic acids during storage and transportation.
Virus Preservation Solution (Inactivated, Guanidine, Red) contains pH buffers, guanidine salts, osmotic pressure maintainers, antibiotics, preservatives, phenol red and other components. After representative collected viruses are preserved in this product, nucleic acid (RNA or DNA) can be extracted, while the integrity of cells or viruses is maintained. When nasal and pharyngeal swabs are placed in the virus preservation solution, its guanidine salts, which are common nuclease activity inhibitors, can protect RNA from degradation by RNases and DNA from degradation by DNases. The combined use of multiple antibiotics and preservatives endows the product with strong antibacterial and antifungal effects. Phenol red can be used to monitor and indicate the pH status of the solution. For some specific applications sensitive to phenol red (e.g., certain cell culture or molecular biology experiments), it may be necessary to use phenol red-free preservation solution or culture medium. This product is for research use only and is not suitable for clinical diagnosis or other applications.
Operating Procedures (For Reference Only):
1. For different purposes, collect virus samples from the corresponding sites using sampling swabs or other tools.
2. Place the samples in the virus preservation solution, which allows short-term storage and transportation at 2–8°C (no more than 48 hours).
Precautions:
1. When handling nasal discharge or sputum specimens, ensure thorough dilution and mixing.
2. The collected specimens must be kept fresh to prevent cell autolysis.
3. This reagent is slightly irritating to the eyes and skin. If it accidentally splashes into the eyes, rinse immediately with clean water. Always operate in a well-ventilated area.
4. This product is for research use only and is not suitable for clinical diagnosis or other applications.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.