细菌的生化试验 (也称生化反应) 是指由于不同细菌具有各自的酶系统，对底物的分解能力不同，由此产生的代谢产物也不同，通过生物化学的方法测定这些代谢产物的过程。生化试验主要包括碳水化合物的生化试验、氨基酸和蛋白质的代谢试验、碳源和氮源的利用试验、酶类的代谢试验等。不同的细菌对蛋白质的分解能力不同，一般先由胞外酶把蛋白质分解为短肽或氨基酸，侵入细菌体内后由胞内酶把肽类分解为氨基酸。
阿拉丁 V.P 试剂的原理是在某些微生物能将葡萄糖分解为丙酮酸，丙酮酸缩合、脱酸转变为乙酰甲基甲醇，在强酸下后者与氧产生二乙酰，二乙酰与蛋白质中的含胍基成分作用生成红色化合物，即为 V.P 实验阳性。V.P 试剂有效成分为萘酚，适用于肠杆菌科细菌、非发酵菌、寄养性细菌、厌氧菌的鉴定。该试剂仅用于科研领域，不宜用于临床诊断或其他用途。
产品组分及储存条件：
V1510397
| Component
| 2×10mL
| 2×50mL
| Storage
|
V1510397A
| V.P 显色液
| 10mL
| 50mL
| RT. Store in the dark.
|
| V1510397B | V.P 碱性基液
| 10mL
| 50mL
| RT |
自备材料：
1、试管或离心管
2、葡萄糖蛋白胨水培养基
3、接种环
4、恒温培养箱
操作步骤(仅供参考)：
1、将待检细菌接种于葡萄糖蛋白胨水培养基，置于恒温培养箱培养。
2、取培养液，加入 V.P 碱性基液，摇匀试管或离心管，加入等量的 V.P 显色液，用力震荡。
3、静置，出现红色为阳性，无色为阴性。
染色结果：
红色：阳性
颜色无变化：阴性
注意事项：
1、培养细菌时，注意避免污染。
2、各试剂注意密闭保存，一旦开封，保质期就会大大缩短。
3、V.P 试剂仅用于科研目的，不可用于临床诊断。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、本产品仅由于科研，严禁他用。
Bacterial biochemical tests (also known as biochemical reactions) refer to the process of determining metabolic products via biochemical methods. This is based on the fact that different bacteria possess distinct enzyme systems, leading to varied abilities to decompose substrates and thus producing different metabolic products. Biochemical tests mainly include carbohydrate biochemical tests, amino acid and protein metabolism tests, carbon and nitrogen source utilization tests, and enzyme metabolism tests. Different bacteria vary in their protein-decomposing capacities. Generally, extracellular enzymes first break down proteins into short peptides or amino acids; after these substances enter bacterial cells, intracellular enzymes further decompose the peptides into amino acids.
The principle of Aladdin V.P. Reagent is as follows: certain microorganisms can decompose glucose into pyruvic acid. Pyruvic acid undergoes condensation and decarboxylation to form acetoin. Under strong acidic conditions, acetoin reacts with oxygen to produce diacetyl, which then interacts with guanidine-containing components in proteins to form a red compound, indicating a positive V.P. test result. The active ingredient of this reagent is naphthol. It is suitable for the identification of Enterobacteriaceae, non-fermenting bacteria, parasitic bacteria, and anaerobic bacteria. This reagent is intended for research purposes only and shall not be used for clinical diagnosis or any other applications.
Product Components and Storage Conditions:
V1510397
| Component
| 2×10mL
| 2×50mL
| Storage
|
V1510397A
| V.P Chromogenic Solution
| 10mL
| 50mL
| RT. Store in the dark.
|
| V1510397B | V.P Alkaline Base Solution
| 10mL
| 50mL
| RT |
Self-prepared Materials:
1. Test tubes or centrifuge tubes
2. Glucose peptone water medium
3. Inoculating loops
4. Constant temperature incubator
Operating Procedures (For Reference Only):
1. Inoculate the test bacteria into the glucose peptone water medium and incubate in a constant temperature incubator.
2. Take the culture solution, add the V.P alkaline base solution, shake the test tube or centrifuge tube thoroughly, then add an equal volume of V.P chromogenic solution and shake vigorously.
3. Allow the mixture to stand. The appearance of a red color indicates a positive result; a colorless mixture indicates a negative result.
Test Results:
Red: Positive
No color change: Negative
Precautions:
1. Avoid contamination during bacterial incubation.
2. All reagents should be stored in sealed containers. Once opened, their shelf life will be significantly shortened.
3. The V.P reagent is intended for research purposes only and shall not be used for clinical diagnosis.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. This product is strictly limited to research use, and any other applications are prohibited.
ZJ26F0333462
危险品化学品经营许可证（带存储）