病毒是一类体积极其微小，能够通过滤菌器的只能在活细胞内生长增殖的微生物，病毒颗粒一般在10～30nm，主要由核酸和蛋白质组成。普通光镜下某些病毒感染的细胞内可见大小和数量不等的圆形或不规则小体，称为病毒包涵体，该物质多位于细胞质内，呈酸性，如狂犬病毒包涵体；有些位于细胞核内，呈碱性，如腺病毒核内包涵体；有些既在细胞质内也在细胞核内，如麻疹病毒包涵体；RNA病毒常形成细胞质内包涵体，DNA病毒多形成细胞核内包涵体。
阿拉丁病毒包涵体染色液采用Mann亚甲蓝伊红染色法，细胞核被亚甲蓝染成蓝色，包涵体被伊红染成红色，在病毒感染中包涵体可能是病毒增殖部位，但应注意并非细胞内的所有包涵体都是病毒，细胞变性也会形成包涵体。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
V1510434
| Component
| 2×20mL
| Storage
|
V1510434A
| 亚甲蓝伊红染色液
| 20mL
| RT
|
V1510434B
| Mann分化液
| 20mL
| RT |
自备材料：
10%中性福尔马林固定液、蒸馏水、系列乙醇
操作步骤(仅供参考)：
1、常规固定 (常采用10%福尔马林)，常规脱水包埋。
2、组织切片二甲苯或脱蜡透明液脱蜡入蒸馏水。
3、入亚甲蓝伊红染色液浸染8～24h，蒸馏水洗2次。
4、Mann分化液分化20～30s。
5、丙酮或乙醇脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
注意事项：
1、病毒很小，光镜下不易被发现，应仔细观察。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用，以防影响后续实验效果。
Viruses are a class of extremely small microorganisms that can pass through bacteriological filters and only grow and replicate inside living cells. Viral particles generally range from 10 to 30 nm in size and are mainly composed of nucleic acids and proteins. Under an ordinary optical microscope, round or irregular small bodies of varying sizes and quantities can be observed in cells infected by certain viruses, which are called viral inclusion bodies. Most of these substances are located in the cytoplasm and are acidic, such as rabies virus inclusion bodies; some are located in the cell nucleus and are alkaline, such as adenovirus intranuclear inclusion bodies; others exist in both the cytoplasm and nucleus, such as measles virus inclusion bodies. In general, RNA viruses tend to form cytoplasmic inclusion bodies, while DNA viruses mostly form intranuclear inclusion bodies.
Aladdin Viral Inclusion Body Staining Solution adopts the Mann methylene blue-eosin staining method. With this solution, cell nuclei are stained blue by methylene blue, and inclusion bodies are stained red by eosin. In viral infections, inclusion bodies may be the sites of viral replication. However, it should be noted that not all inclusion bodies in cells are viral in origin—cellular degeneration can also lead to the formation of inclusion bodies. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
V1510434
| Component
| 2×20mL
| Storage
|
V1510434A
| Methylene Blue-Eosin Staining Solution
| 20mL
| RT
|
V1510434B
| Mann Differentiating Solution
| 20mL
| RT |
Materials to Be Prepared:
10% neutral formalin fixative, distilled water, graded ethanol solutions
Operating Procedures (For Reference Only):
1. Routine Fixation and Embedding: Fix the sample routinely (10% formalin is commonly used), followed by routine dehydration and embedding.
2. Deparaffinization and Hydration: Deparaffinize the tissue sections with xylene or deparaffinizing clearing solution, and then hydrate them to distilled water.
3. Staining: Immerse the sections in methylene blue-eosin staining solution for 8–24 hours, followed by rinsing twice with distilled water.
4. Differentiation: Differentiate the sections with Mann differentiating solution for 20–30 seconds.
5. Dehydration, Clearing and Mounting: Dehydrate the sections with acetone or ethanol, clear them with xylene or deparaffinizing clearing solution, and mount the sections with neutral balsam.
Staining Results:
Inclusion bodies
| Red
|
Cell nuclei
| Blue
|
Precautions:
1. Viruses are extremely small and difficult to detect under a light microscope, so careful observation is required.
2. For your safety and health, wear a lab coat and disposable gloves during the operation.
3. Use the reagents as soon as possible after opening to avoid affecting the results of subsequent experiments.
ZJ26F0333471
危险品化学品经营许可证（带存储）