病毒是一类体积极其微小，能够通过滤菌器的只能在活细胞内生长增殖的微生物，病毒颗粒一般在10～30nm，主要由核酸和蛋白质组成。普通光镜下某些病毒感染的细胞内可见大小和数量不等的圆形或不规则小体，称为病毒包涵体，该物质多位于细胞质内，呈酸性，如狂犬病毒包涵体；有些位于细胞核内，呈碱性，如腺病毒核内包涵体；有些既在细胞质内也在细胞核内，如麻疹病毒包涵体；RNA病毒常形成细胞质内包涵体，DNA病毒多形成细胞核内包涵体。
阿拉丁病毒包涵体染色液采用Lendrum法，该法又称荧光桃红-酒石黄法，细胞核被染成蓝灰色，包涵体被染成红色，在病毒感染中包涵体可能是病毒增殖部位，但应注意并非细胞内的所有包涵体都是病毒，细胞变性也会形成包涵体。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
V1510438
| Component
| 3×20mL
| Storage
|
V1510438A
| Lea苏木素染色液
| 20mL
| RT
|
V1510438B
| 荧光桃红染色液
| 20mL
| RT. Store in the dark.
|
V1510438C
| 酒石黄染色液
| 20mL
| RT. Store in the dark. |
自备材料：
10%中性福尔马林固定液、蒸馏水、系列乙醇
操作步骤(仅供参考)：
1、常规固定 (常采用10%中性福尔马林固定液)，常规脱水包埋。
2、常规二甲苯或脱蜡透明液脱蜡至水。
3、入Lea苏木素染色液浸染10min，自来水冲洗5min。
4、入荧光桃红染色液浸染20min，自来水冲洗，吸干玻片。
5、入酒石黄染色液浸染，显微镜下控制染色，当被染成鲜红色时停止染色，一般需要5～10min。
6、95%乙醇冲洗。
7、丙酮或乙醇脱水，常规二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
包涵体
| 鲜红色
|
细胞核
| 蓝灰色
|
红细胞
| 橙色至红色不等
|
背景
| 黄色
|
注意事项：
1、病毒很小，光镜下不易被发现，应仔细观察。
2、所有的组织都会被荧光桃红染成红色，通过酒石黄复染，部分红色被替换出来，从而将组织区分开来，首先褪去红色的是肌肉组织，随后是其他结缔组织。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
4、试剂开封后请尽快使用，以防影响后续实验效果。
Viruses are a class of extremely small microorganisms that can pass through bacteriological filters and only replicate and proliferate inside living cells. Viral particles generally range from 10 to 30 nm in size and are mainly composed of nucleic acids and proteins. Under a conventional light microscope, round or irregular small bodies of varying sizes and quantities can be observed in cells infected by certain viruses, which are called viral inclusion bodies. Most of these structures are located in the cytoplasm and are acidic, such as rabies virus inclusion bodies; some are located in the cell nucleus and are alkaline, such as adenovirus intranuclear inclusion bodies; others exist in both the cytoplasm and nucleus, such as measles virus inclusion bodies. In general, RNA viruses tend to form cytoplasmic inclusion bodies, while DNA viruses mostly form intranuclear inclusion bodies.
Aladdin Viral Inclusion Body Stain Solution adopts the Lendrum method, also known as the phloxine-tartrazine method. With this solution, cell nuclei are stained blue-gray and inclusion bodies are stained red. In viral infections, inclusion bodies may be the sites of viral replication. However, it should be noted that not all inclusion bodies in cells are of viral origin—cellular degeneration can also lead to the formation of inclusion bodies. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
V1510438
| Component
| 3×20mL
| Storage
|
V1510438A
| Lea Hematoxylin Staining Solution
| 20mL
| RT
|
V1510438B
| Phloxine Staining Solution
| 20mL
| RT. Store in the dark.
|
V1510438C
| Tartrazine Staining Solution
| 20mL
| RT. Store in the dark. |
Materials to Be Prepared:
10% neutral formalin fixative, distilled water, graded ethanol solutions
Operating Procedures (For Reference Only):
1. Routine Fixation and Embedding: Fix the sample routinely (10% neutral formalin fixative is commonly used), followed by routine dehydration and embedding.
2. Routine Deparaffinization to Water: Deparaffinize the sections to water using xylene or deparaffinizing clearing solution following standard protocols.
3. Hematoxylin Staining: Immerse the sections in Lea hematoxylin staining solution for 10 minutes, then rinse with tap water for 5 minutes.
4. Phloxine Staining: Immerse the sections in phloxine staining solution for 20 minutes, rinse with tap water, and blot the slides dry.
5. Tartrazine Staining: Immerse the sections in tartrazine staining solution. Monitor the staining process under a microscope and stop when the target structures turn bright red, which usually takes 5–10 minutes.
6. Rinsing: Rinse the sections with 95% ethanol.
7. Dehydration, Clearing and Mounting: Dehydrate the sections with acetone or ethanol, clear them with xylene or deparaffinizing clearing solution following standard procedures, and mount the sections with neutral balsam.
ZJ26F0333472
危险品化学品经营许可证（带存储）