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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
维生素C(Vitamin C)又称L-抗坏血酸,是高等灵长类动物与其他少数生物的必需营养素。在生物体内,维生素C是一种抗氧化剂,为酸性己糖衍生物,是稀醇式己糖酸内酯,保护身体免于自由基的威胁,同时也是一种辅酶,其广泛的食物来源为各类新鲜蔬果。Vc有L-型和D-型两种异构体,只有L-型的才具有生理功能,还原型和氧化型都有生理活性。 该试剂盒的检测原理是铜盐与过量的碘盐形成碘化铜,而碘化铜不稳定,分解为碘化亚铜和游离碘,其释放出来的游离碘能够氧化维生素C形成碘化氢,直到维生素C完全被氧化,剩余的微量碘与淀粉指示剂形成棕红色(或红紫色或蓝色),该法终点明确,简单快速,可准确测量维生素C的含量,其误差一般小于2%。本产品仅用于科研领域,不宜用于临床诊断或其他用途。
Vitamin C, also known as L-ascorbic acid, is an essential nutrient for higher primates and a few other organisms. Within living organisms, vitamin C acts as an antioxidant. It is an acidic hexose derivative—specifically an enol-form hexonic acid lactone—that helps protect the body from threats posed by free radicals and also functions as a coenzyme. It is widely available from various fresh fruits and vegetables. Vitamin C exists in two isomeric forms, L-type and D-type, with only the L-type possessing physiological activity. Both the reduced and oxidized forms are biologically active. The detection principle of this kit is based on the formation of copper iodide from a copper salt and an excess of an iodide salt. The resulting copper iodide is unstable and decomposes into cuprous iodide and free iodine. The released free iodine then oxidizes vitamin C to form hydrogen iodide. This reaction continues until all the vitamin C is completely oxidized. The remaining trace amount of iodine reacts with a starch indicator to produce a reddish-brown (or purplish-red or blue) color. This method offers a clear endpoint, is simple and rapid, and allows for accurate measurement of vitamin C content, with a general error margin of less than 2%. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.