BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
维生素B1(Vitamin B1)又称硫胺素，属于水溶性维生素，在有氧化剂存在时容易被氧化产生脱氢硫胺素，后者在有紫外光照射时呈现蓝色荧光。 该试剂盒是利用Vitamin B1水溶液在碱性盐溶液中能被氧化成蓝色的硫色素(一种荧光化合物)，在紫外线下硫色素发出荧光，在激发波长365nm，发射波长435nm，在没有其他荧光物质干扰时荧光强度与硫色素的浓度呈正比，可定量检测维生素B1的含量，如果样品中存在干扰物质，可用沸石吸附Vitamin B1，以便去除待测样品中干扰荧光测定的杂质。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Vitamin B1, also known as thiamine, is a water-soluble vitamin that is readily oxidized in the presence of oxidizing agents to form thiochrome, which exhibits blue fluorescence under ultraviolet light. The detection principle of this kit is based on the oxidation of Vitamin B1 in an alkaline salt solution to form thiochrome, a fluorescent compound. Under ultraviolet light, thiochrome emits fluorescence, with an excitation wavelength of 365 nm and an emission wavelength of 435 nm. In the absence of interfering fluorescent substances, the fluorescence intensity is proportional to the concentration of thiochrome, enabling the quantitative determination of Vitamin B1 content. If interfering substances are present in the sample, zeolite can be used to adsorb Vitamin B1, thereby removing impurities that may interfere with the fluorescence measurement. This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
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