计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
维生素B2(Vitamin B2)又称核黄素,是一种异咯嗪衍生物,耐热、微溶于水,中性水溶液及乙醇溶液呈黄色,具有很强的荧光,在pH值6~7的溶液中荧光最强,在pH=11时荧光消失,核黄素可被还原性物质还原为无色的二氢化物,同时失去荧光;二氢化物在空气中易重新氧化,恢复其荧光。 该试剂盒是利用Vitamin B2的中性溶液有强荧光,当加入有还原性的亚硫酸盐时,核黄素被还原为无荧光的物质,根据稀核黄素溶液在还原前后荧光强度的变化即可定量检测Vitamin B2的含量,其激发波长为440~500nm(多为460nm),发射波长为510~550nm(多为520nm)。本产品仅用于科研领域,不适用于临床诊断或其他用途。
Vitamin B2, also known as riboflavin, is an isoalloxazine derivative. It is heat-stable, slightly soluble in water, and its neutral aqueous or ethanol solutions appear yellow. It exhibits strong fluorescence, which is most intense in solutions with a pH of 6–7 and disappears at pH 11. Riboflavin can be reduced by reducing agents to a colorless dihydro compound, simultaneously losing its fluorescence. The dihydro compound is readily reoxidized in air, restoring its fluorescence. The detection principle of this kit is based on the strong fluorescence of Vitamin B2 in neutral solutions. When a reducing sulfite is added, riboflavin is reduced to a non-fluorescent substance. The Vitamin B2 content can be quantitatively determined by measuring the change in fluorescence intensity of a dilute riboflavin solution before and after reduction. The excitation wavelength ranges from 440 to 500 nm (typically 460 nm), and the emission wavelength ranges from 510 to 550 nm (typically 520 nm). This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.