计算溶液所需的质量、体积或浓度。
Vero无血清培养基 (不含酚红, 含谷氨酰胺)
有货
为什么选择此级别
BioReagent,无菌过滤,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
是否无菌：是
Vero无血清培养基是哺乳动物传代细胞系通用型贴壁培养基，广泛应用于疫苗贴壁生产。
培养基适应
直接适应：最初培养阶段，细胞按初始密度1/3 传代，直接将培养基更换为Aplo VVP1，进行细胞培养。待细胞培养2-3 代，且细胞生长稳定后进行后续实验。贴壁细胞稳定生长时按照1/3 传代，72 小时显微镜下观察细胞生长良好，贴壁单层且汇合度在80%左右。
间接适应：Aplo VVP1 与细胞原培养基1：1 比例混合，培养细胞，连续传代2-3 代，细胞稳定生长后可将培养基更换为Aplo VVP1，连续传代2-3 代，细胞生长稳定后进行后续实验。贴壁细胞稳定生长时按照1/3 传代，72 小时显微镜下观察细胞生长良好，贴壁单层且汇合度在80%左右。
注意事项
1.根据细胞具体情况选择进行培养基直接适应或者间接适应。
2.细胞驯化初期，若出现细胞汇合度较低、细胞漂浮等其他不稳定状态，根据当天细胞生长情况选择传代方式（换液传代、离心传代）。
3.换液传代：若细胞汇合度≥30%，弃去旧细胞悬液，加入对应新鲜培养基。
4.离心传代：若细胞汇合度＜30%，收集全部细胞悬液，进行800rpm 离心去除上清，用新鲜培养基重悬细胞，补加对应新鲜培养基。
5.细胞恢复正常状态传代：贴壁细胞按照1/3 传代，72h 显微镜下观察细胞生长良好，贴壁单层且汇合度在80%左右。
贴壁细胞冻存
1.待细胞恢复至正常状态后，扩大细胞培养体积准备冻存建库。尽量多建细胞库，保证备份充足。
2.选择处于对数生长期、活率大于90%细胞进行冻存，冻存细胞推荐1 个T75冻存3～4 支。
3.准备冻存盒：向程序降温盒注入适量异丙醇，置于4℃冰箱预冷。取细胞悬液，800rpm 离心5min，弃去上清，用适量细胞冻存液重悬细胞，
4.调整细胞密度至目标值。
5.快速分装细胞液至冻存管中。
6.将冻存管放入程序降温盒中，放于-80℃冰箱，24h 后转至液氮罐中储存。一段时间后复苏检测。
贴壁细胞复苏
1.预热培养基：取15mL 对应培养基置于方瓶中，放入37℃二氧化碳培养箱中预热30min 以上（平衡培养基pH 值），保证预热充分。
2.将冻存管从液氮保存罐或-80℃冰箱中取出，迅速转移至37℃水浴中，快速摇晃，直至完全融化。
3.取预热好的培养基5mL 于无菌离心管中，并将溶化后的细胞悬液移入此离心管中。800rpm 离心5min（除去冻存液中DMSO）。
4.离心后去除上清，用10mL 预热好的培养基（保证较高细胞复苏密度）重悬细胞，移回相应方瓶，放于培养箱中培养。
Vero serum-free medium is a universal adherent medium for mammalian cell lines, widely used in adherent vaccine production.
Medium Adaptation
Direct adaptation: In the initial culture phase, cells are passaged at a split ratio of 1:3, and the medium is directly replaced with Aplo VVP1 for culture. After 2–3 passages, when cell growth is stable, subsequent experiments can be carried out. For stable adherent cell growth, cells are passaged at 1:3; after 72 hours, microscopic observation should show good cell growth, an adherent monolayer, and a confluence of approximately 80%.
Indirect adaptation: Aplo VVP1 and the original cell medium are mixed at a 1:1 ratio for culture. Cells are passaged continuously for 2–3 generations. After stable growth is achieved, the medium is replaced with Aplo VVP1, and cells are passaged for another 2–3 generations. Once growth is stable, subsequent experiments can be performed. For stable adherent cell growth, cells are passaged at 1:3; after 72 hours, microscopic observation should show good cell growth, an adherent monolayer, and a confluence of approximately 80%.
Precautions
1.Choose direct or indirect adaptation based on the specific condition of the cells.
2.During the early stage of cell acclimatization, if unstable conditions such as low confluence or floating cells occur, select the appropriate subculture method (medium‑change subculture or centrifugation subculture) based on the day’s cell growth status.
3.Medium‑change subculture: If confluence is ≥30%, discard the old cell suspension and add fresh corresponding medium.
4.Centrifugation subculture: If confluence is <30%, collect the entire cell suspension, centrifuge at 800 rpm to remove the supernatant, resuspend the cells in fresh medium, and replenish with fresh corresponding medium.
5.Normal cell subculture after recovery: Adherent cells are passaged at a 1:3 ratio; after 72 h, microscopic observation should show good cell growth, an adherent monolayer, and a confluence of approximately 80%.
Cryopreservation of Adherent Cells
1.After cells return to normal status, expand the culture volume for cryopreservation and banking. Prepare as many cell banks as possible to ensure sufficient backups.
2.Select cells in logarithmic growth phase with viability >90% for cryopreservation. It is recommended to cryopreserve 3–4 vials per T75 flask.
3.Prepare the freezing container: Add an appropriate amount of isopropanol to the controlled‑rate freezing container and pre‑cool at 4°C.
4.Collect the cell suspension, centrifuge at 800 rpm for 5 min, discard the supernatant, and resuspend the cells in an appropriate volume of cryopreservation medium to adjust to the target density.
5.Quickly aliquot the cell suspension into cryovials.
6.Place the cryovials into the controlled‑rate freezing container, store at –80°C for 24 h, then transfer to liquid nitrogen. After a period of time, thaw one vial for testing.
Thawing of Adherent Cells
1.Pre‑warm medium: Place 15 mL of the corresponding medium in a square flask and pre‑warm in a 37°C CO₂ incubator for at least 30 min (to equilibrate the pH) to ensure thorough pre‑warming.
2.Remove the cryovial from liquid nitrogen or –80°C storage and quickly transfer to a 37°C water bath with rapid shaking until completely thawed.
3.Transfer 5 mL of pre‑warmed medium into a sterile centrifuge tube, then transfer the thawed cell suspension into this tube. Centrifuge at 800 rpm for 5 min (to remove DMSO).
规格
英文别名
Vero SFM（Serum-Free Medium）
规格或纯度
无菌过滤, BioReagent, 用于细胞培养, 内毒素 ＜2 EU/mL; 渗透压 280-320 mOsm/kg
稳定性与储存
Store at 2-8℃ long term (12 months). Avoid freeze/thaw cycle.
英文名称
Vero Serum-Free Medium (without phenol red, with glutamine)
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
产品属性
| pH | 7.0-7.4 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0939083 |
分析证书 | V1525346 |
溶液计算器
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Sterile-filtered 级别指南 → 查看 BioReagent 级别指南 → 查看 for Cell culture 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）