BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
该试剂盒采用pH 9.6～9.7的碱性缓冲液提取样品中的硝酸根离子，提取过程中加入活性炭以去除色素类物质，并加入沉淀剂以去除蛋白质及其他干扰物质。利用硝酸根离子与亚硝酸根离子在紫外区219 nm处具有等吸收波长的特性，测定提取液的吸光度，所得结果为硝酸盐与亚硝酸盐吸光度的总和。鉴于新鲜蔬菜、水果中亚硝酸盐含量甚微，可忽略不计，故该测定结果可视为硝酸盐的吸光度。依据标准曲线即可计算样品中的硝酸盐含量。
This kit employs an alkaline buffer at pH 9.6–9.7 to extract nitrate ions from samples. During the extraction process, activated carbon is added to remove pigments, and a precipitant is added to eliminate proteins and other interfering substances. Taking advantage of the property that nitrate and nitrite ions exhibit equal absorbance at 219 nm in the ultraviolet region, the absorbance of the extract is measured. The result obtained is the combined absorbance of nitrate and nitrite. Since the nitrite content in fresh vegetables and fruits is extremely low and negligible, this measured value can be regarded as the absorbance attributable to nitrate. The nitrate content in the sample is then calculated based on a standard curve.
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