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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
维生素B₁（Vitamin B₁）是构成脱羧辅酶（焦磷酸硫胺素，TPP）的主要成分，参与糖代谢中α-酮酸脱羧反应及三羧酸循环，是维持机体正常代谢所必需的水溶性维生素，在生物体能量代谢中发挥重要作用。本试剂盒的测定原理为：在碱性条件下，维生素B₁还原铁氰化钾生成亚铁氰化钾；亚铁氰化钾与Fe³⁺在弱酸条件下反应生成普鲁士蓝，该产物在704 nm处具有特征吸收峰。通过测定704 nm处吸光度，即可定量计算样品中维生素B₁的含量。
Vitamin B₁ is the principal constituent of decarboxylase coenzyme (thiamine pyrophosphate, TPP). It participates in the decarboxylation of α-keto acids and the tricarboxylic acid cycle during carbohydrate metabolism. As an essential water-soluble vitamin for sustaining normal bodily metabolism, it plays a vital role in energy metabolism of organisms. The detection principle of this kit is as follows: under alkaline conditions, vitamin B₁ reduces potassium ferricyanide to potassium ferrocyanide. Potassium ferrocyanide reacts with ferric ions under weak acidic conditions to produce Prussian blue, which possesses a characteristic absorption peak at 704 nm. The content of vitamin B₁ in samples can be quantitatively calculated by measuring the absorbance at 704 nm.
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