是否无菌：是
Versene 溶液是一种温和的、即用型非酶类细胞消化液，主要成分为 0.02% EDTA·Na₄（乙二胺四乙酸四钠），相当于 0.48 mM，配制于无钙、无镁的磷酸盐缓冲液（PBS） 中。本品不含动物源性物质、不含酚红，化学成分明确，批间稳定性高，适用于对生物安全性及成分明确性要求严格的实验体系。
作用原理
EDTA 是一种金属离子螯合剂，能够特异性螯合 Ca²⁺、Mg²⁺ 等二价阳离子。Ca²⁺ 和 Mg²⁺ 是维持细胞间黏附连接的重要因子，EDTA 通过竞争性结合这些离子，破坏细胞间连接，促使细胞从贴壁状态分散为单细胞悬液。
产品特点
• 作用温和：不破坏细胞表面蛋白分子，尤其适合对膜蛋白完整性要求较高的下游实验。
• 操作简便：即开即用，无需配制，毒性低。
• 兼容性好：可与胰蛋白酶按比例混合使用，也可作为酶消化前的细胞洗涤液。
• 成分安全：无动物源成分、无酚红，减少实验变量干扰。
适用范围
Versene 溶液适用于哺乳动物细胞的温和解离，尤其适合要求保留完整细胞表面蛋白的研究场景，包括但不限于：细胞膜受体检测、流式细胞分选（FACS）、免疫荧光染色（IF）、免疫组化（IHC）；
本品也可用于：胰蛋白酶处理前对细胞的预洗涤；与胰蛋白酶按比例配合使用，调节消化强度。
适用细胞类型
适用于贴壁能力较弱的细胞系（如 293T、KATO Ⅲ 等）。
不推荐用于贴壁能力强、细胞间连接紧密的细胞系（如 HeLa、MDA‑MB‑231 等）的常规传代培养。
使用方法
1. 取适量 Versene 溶液置于 37℃进行预热。
2. 吸去培养液，用不含 Ca²⁺和 Mg²⁺的无菌 PBS 或 D-PBS 洗涤细胞 2 次。
3. 加入适量 Versene 溶液（一般每 25 cm² 加入约 1.5 mL Versene 溶液），室温放置 2–20 min，可轻轻摇晃培养皿或培养瓶。
注：不同的细胞消化时间有所不同，若细胞贴壁比较牢固难以消化，可适当增加处理时间或 Versene 溶液的用量。
4. 显微镜下观察，细胞明显收缩，并且肉眼观察培养器皿底部发现细胞形态发生明显变化；或者用移液枪吹打细胞，发现细胞刚好可以被吹打下来。此时吸除 Versene 溶液。加入适量 PBS 或 D-PBS，吹打下细胞，1000×g 离心 1 min 后用适量培养液重悬，即可直接用于后续实验。
5. 如果消化不足，可加入 Versene 溶液重新消化。
6. 如果发现细胞消化时间过长，则直接把细胞全部吹打下来。1000-2000×g 离心 1 min，沉淀细胞，尽量去除 Versene 溶液后，用 PBS 洗涤一次，加入含血清的完全培养液重新悬浮细胞，即可用于后续实验。
注意事项
1、本产品已过滤除菌，且不含抑菌剂，操作时严格无菌操作，防止污染。
2、本品解离作用温和，不推荐用于高贴壁细胞的常规传代。
3、不同细胞生物学特性存在差异，实验人员需摸索最优消化时间；消化不宜过久，否则会损害细胞贴壁能力与生长状态。
4、EDTA 无法被培养基中和，消化收集的细胞建议使用 PBS 洗涤一次，避免后续细胞难以贴壁。
5、本产品仅供科研专业人员开展科学研究使用，不可用于临床诊断与治疗，不可作为食品、药品相关原料，请勿存放于居民住宅。
6、实验操作时，请穿戴实验服与一次性手套，做好个人防护。
Versene Solution is a mild, ready-to-use, non-enzymatic cell dissociation reagent. Its main component is 0.02% EDTA·Na₄ (tetrasodium ethylenediaminetetraacetate), equivalent to 0.48 mM, prepared in calcium- and magnesium-free phosphate-buffered saline (PBS).
This product is animal-origin-free and phenol-red-free, with a chemically defined formulation and high batch-to-batch consistency. It is suitable for experimental systems that require stringent standards for biological safety and compositional clarity.
Mechanism of Action
EDTA is a metal ion chelator that specifically chelates divalent cations such as Ca²⁺ and Mg²⁺. Ca²⁺ and Mg²⁺ are essential factors for maintaining cell-cell adhesion. By competitively binding these ions, EDTA disrupts intercellular junctions, promoting the dissociation of adherent cells into single-cell suspensions.
Product Features
• Gentle action: Does not damage cell surface proteins, making it particularly suitable for downstream applications that require intact membrane proteins.
• Easy to use: Ready-to-use solution, no preparation required, low cytotoxicity.
• Good compatibility: Can be used in combination with trypsin at appropriate ratios, or as a pre-wash solution prior to enzymatic digestion.
• Safe composition: Free of animal-derived components and phenol red, minimizing experimental variables.
Applications
Versene Solution is suitable for gentle dissociation of mammalian cells, particularly in research scenarios that require preservation of intact cell surface proteins, including but not limited to:
• Cell surface receptor assays
• Fluorescence-activated cell sorting (FACS)
• Immunofluorescence staining (IF)
• Immunohistochemistry (IHC)
This product can also be used for:
• Pre-washing cells prior to trypsin treatment
• Combination with trypsin at appropriate ratios to modulate digestion intensity
Suitable Cell Types
Suitable for weakly adherent cell lines (e.g., 293T, KATO Ⅲ).
Not recommended for routine subculture of strongly adherent cell lines with tight intercellular junctions (e.g., HeLa, MDA-MB-231).
Protocol
1. Pre-warm an appropriate volume of Versene Solution at 37℃.
2. Aspirate culture medium, and wash the cell monolayer twice with sterile PBS or D-PBS without Ca²⁺ and Mg²⁺.
3. Add appropriate volume of Versene Solution (typically 1.5 mL for every 25 cm² culture area). Incubate at room temperature for 2–20 min; gently rock the dish or flask occasionally.
Note: Optimal incubation time varies between cell types. For tightly adherent cells, extend incubation time or increase the volume of Versene Solution.
4. Observe cells under microscope: cells show obvious rounding, and visible morphological changes can be seen at the bottom of culture vessel. Alternatively, test by gentle pipetting; proceed when cells can just be dislodged. Aspirate Versene Solution at this stage. Add PBS or D-PBS, pipette to harvest cells, centrifuge at 1000×g for 1 min, then resuspend cell pellet with culture medium for subsequent experiments.
5. If dissociation is insufficient, add Versene Solution and repeat incubation.
6. If cells have been incubated for excessive dissociation time, gently pipette to harvest all cells directly. Centrifuge at 1000–2000×g for 1 min to pellet cells. Remove Versene Solution as thoroughly as possible, wash the cell pellet once with PBS, and resuspend cells in serum-supplemented complete culture medium for subsequent experiments.
Precautions
1. This product is sterile filtered and contains no preservatives. Strict aseptic operation is required during use to prevent contamination.
2. This product features mild dissociation performance and is not recommended for routine passaging of strongly adherent cell lines.
3. Due to differences among cell types, operators shall optimize the incubation time empirically. Over-digestion will impair cell adhesion and proliferation.
4. EDTA cannot be neutralized by culture medium. Harvested cells should be washed once with PBS to avoid poor cell attachment.
5. This product is for research use only by qualified professionals. It must not be applied for clinical diagnosis or therapy, must not be used in food or pharmaceutical production, and should not be stored in residential premises.
6. Wear a lab coat and disposable gloves for safe handling.
危险品化学品经营许可证（带存储）