计算溶液所需的质量、体积或浓度。
BioReagent,无菌 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品专门用于冠状病毒、流感病毒、手足口病毒、麻疹风疹病毒等各种病毒及相关样本的采集和常温运输,方便样品的运输和异地检测。
本产品具有以下特点:
1. 含病毒灭活试剂,反应快速,可以在数分钟之内灭活病毒 (对各类 DNA 病毒和 RNA 病毒均有效),使其失去传染性。
2. 含有高效酶灭活试剂和特殊的核酸保护剂,可以快速灭活包括 DNase 和 RNase 在内的各种核酸酶,去除降解核酸的隐患,确保常温条件下 4 天内核酸无明显降解。如果超过 4 天需-20℃保存和运输。。
3. 适用于各种拭子的采集保存和运输,包括口腔拭子、咽喉拭子、鼻腔拭子、阴道拭子等。
4. 保存的样品可以直接用于后续的核酸纯化及检测。
5. 本产品保存液中的病毒已灭活,不能用于病毒培养。
6. 本产品只能用于科研。
7. 本产品为 10 倍的病毒保存液浓缩液,需使用超纯水稀释 10 倍,使用稀释液保存样本。
使用方法
注意:尽管本产品含有病毒灭活剂,但不能排除离心管外在取样时污染了病毒,因此操作人员必须按处理传染性样品的方法进行相应的防护,必须在合乎国家规定的实验室进行所有操作。本产品具有抑菌性,但不能排除分装到采样管中时不被污染,分装前后建议进行适当的灭菌处理。
1. 先将本产品稀释 10 倍,得到 1× 保存液。如若需要 10L 的病毒保存液,取 1L 本产品,加入 9L 超纯水充分搅拌混匀即可。
2. 稀释好的 1× 保存液进行分装,管内装量:3ml (一拖一),6ml (一拖五,一拖十)。
3. 采样前,在含有病毒保存液的采样管的标签上标注相关样品信息。
4. 根据不同的采样要求,用采样拭子在相应的部位和器官采样。具体采样方法如下:
a) 鼻拭子:将拭子头轻轻插入鼻道内鼻腭处,停留片刻后缓慢转动退出。
b) 咽拭子:用拭子擦拭双侧咽扁桃体及咽后壁后退出。
c) 尿道采样:对男性,用拭子插入尿道约 2cm 处旋转,静止数秒后退出。对女性,抹去宫颈口粘液,用拭子插入宫颈管 1-2cm 处后退出。
d) 其他拭子采样:本手册暂不详述。
5. 迅速将拭子放入含有病毒保存液的采样管中,将拭子在样品管中旋转 15 秒。
6. 将采样拭子在折断处折断,丢弃把柄端,旋紧管盖。
7. 新鲜采集的标本应在 4℃-25℃下 96 小时 (4 天) 内运送至实验室,未能 96 小时送至实验室的,应置于-20℃保存。
This product is specially designed for the collection and ambient‑temperature transportation of samples containing coronavirus, influenza virus, hand‑foot‑and‑mouth virus, measles‑rubella virus and other viruses, facilitating sample transport and off‑site testing.
This product features the following characteristics:
1. It contains virus‑inactivating reagents with rapid reaction speed, which can inactivate viruses (effective against all types of DNA and RNA viruses) within several minutes to render them non‑infectious.
2. It contains highly‑effective nuclease‑inactivating reagents and special nucleic acid protectants, which can rapidly inactivate various nucleases including DNase and RNase to eliminate the risk of nucleic acid degradation. It ensures no significant degradation of nucleic acids within 4 days at ambient temperature. Samples stored for more than 4 days shall be stored and transported at ‑20 °C.
3. Suitable for the collection, preservation and transportation of samples collected with various swabs, including oral swabs, throat swabs, nasal swabs, vaginal swabs, etc.
4. Preserved samples can be directly used for subsequent nucleic acid purification and testing.
5. Viruses in samples preserved with this product have been inactivated; the samples cannot be used for virus culture.
6. This product is for research‑only use.
7. This product is a 10‑fold concentrated virus preservation solution. It shall be diluted 10‑fold with ultrapure water, and the diluted solution shall be used for sample preservation.
Instructions for Use:
Note: Although this product contains virus‑inactivating agents, viral contamination on the outer surface of the centrifuge tube during sampling cannot be excluded. Operators must take appropriate protective measures as required for handling infectious samples, and all operations must be performed in laboratories complying with national regulations. This product has bacteriostatic properties; however, contamination during aliquoting into sampling tubes cannot be completely ruled out. Appropriate sterilization treatment is recommended before and after aliquoting.
1. Dilute this product 10‑fold to prepare 1× preservation solution. For example, to prepare 10 L of virus preservation solution, take 1 L of this product and add 9 L of ultrapure water, then stir thoroughly to obtain a homogeneous mixture.
2. Aliquot the prepared 1× preservation solution. Fill‑volume specification: 3 mL (1‑swab‑1‑tube configuration); 6 mL (5‑swab‑1‑tube and 10‑swab‑1‑tube configurations).
3. Before sampling, label the sampling tube pre‑filled with virus preservation solution with relevant sample information.
4. Collect samples from corresponding sites using sampling swabs according to sampling requirements. Specific sampling procedures are described below:
a) Nasal swab: Gently insert the swab tip into the nasopalatine region of the nasal passage, hold for a moment, rotate slowly and withdraw.
b) Throat swab: Swab both palatine tonsils and the posterior pharyngeal wall with the swab, then withdraw.
c) Urethral sampling: For males, insert the swab approximately 2 cm into the urethra, rotate, hold for several seconds and withdraw. For females, remove mucus at the cervical orifice, insert the swab 1‑2 cm into the cervical canal and withdraw.
d) Other swab sampling methods: Not detailed in this manual.
5. Immediately place the swab into the sampling tube containing virus preservation solution, and rotate the swab inside the tube for 15 seconds.
6. Break the swab at the break‑point, discard the handle segment, and tighten the tube cap.
7. Freshly collected specimens must be transported to the laboratory within 96 hours (4 days) at 4°C–25°C. Samples that cannot be delivered to the laboratory within 96 hours should be stored at -20°C.