计算溶液所需的质量、体积或浓度。
VBS⁺⁺ (with Ca⁺⁺ and Mg⁺⁺)
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
General Description
VBS⁺⁺ is like the traditional buffer used in complement assays (GVB⁺⁺) but it lacks the carrier protein gelatin often used in these assays. GVB may be replaced with VBS if gelatin is undesirable or may interfere with the assays. The concentrations of metal ions are the traditional concentrations and these give maximal complement activation (see Buffer Components sectionbelow). Dilutions of serum and other assay components for CH50 assays may prepared in this VBS⁺⁺ buffer (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).
Buffer Components
Veronal is used as the buffer because in the mid-1900s this was the only buffer for pH range 7.2-7.4 that did not chelate metal ions and did not inhibit complement reactions as did other buffers. Sodium chloride is present to provide an isotonic environment so that cells do not lyse due to osmotic pressure. Azide is present to prevent bacterial growth. Calcium is present because the classical and lectin pathways require it to hold the subunits of the C1, MBL and ficolin complexes together. Magnesium is required for formation of the C3 and C5 convertases of all three pathways of complement.
Although total calcium in plasma and serum is about 2.3 mM, the free available concentration is about 1.07 mM. The remainder is complexed to proteins or small molecules. Complement buffers contain 0.15 mM calcium because it was found that under CH50 assay conditions the classical pathway activity is greater at 0.15 mM than at 1.0 mM calcium. Plasma and serum concentrations of total magnesium are about 0.87 mM and the freeavailable concentration is 0.5 mM. Thus, GVB⁺⁺ contains 0.5 mM magnesium chloride.
Physical Characteristics
The buffer is stable at 4℃ and should be usable for 3 months from the time of manufacture. The salts in this buffer may crystalize if the buffer is frozen. They can be redissolved easily by heating to 37℃ or by brief warming in a microwave oven. The solution should be thoroughly mixed after these procedures. Extended time above 4℃ may shorten the time this buffer will resist bacterial growth.
Applications
VBS⁺⁺ may be used when a buffer free of carrier proteins is needed for diluting proteins or as the buffer for complement assays. VBS⁺⁺ may also be used to prepare GVB°, GVB⁺⁺, and GVBE or it may be used to prepare similar complement buffers that include different carrier proteins in place of gelatin in situations where gelatin interferes with assays. BSA (0.1%) or another carrier protein may be used for this.
规格
产品名称
VBS⁺⁺ (with Ca⁺⁺ and Mg⁺⁺)
规格或纯度
5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, pH 7.3. Containing 0.15 mM calcium chloride and 0.5 mM magnesium chloride
分子类型
生物试剂/缓冲液
产品线
生物产品
储存与运输
储存缓冲液
Sodium veronal
浓度
5 mM Veronal, 145 mM NaCl, 0.025 % NaN3, pH 7.3. Containing 0.15 mM calcium chloride and 0.5 mM magnesium chloride
储存条件
2-8°C储存
运输条件
低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）