计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
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产品简介
本试剂盒适用于从全血、组织匀浆液、拭子，以及血清、血浆等无细胞体液中简 单、快速、高效地分离纯化DNA/RNA。独特的缓冲体系使裂解液中的病毒核酸高效特 异地结合在硅胶质离心吸附柱上，获得的病毒核酸纯度高，质量稳定，不含蛋白、核 酸酶和其他杂质，可适用于各种常规操作，包括PCR、荧光定量PCR等实验。
自备仪器
恒温混匀仪。
实验前准备及重要注意事项
1.在实验前，应仔细阅读本说明书。
2.Proteinase K若需长期保存，请放置于-20℃。
3.使用前请检查Buffer RLC是否出现结晶或者沉淀，如有结晶或者沉淀，请将Buffer RLC于56℃水浴重新溶解。
4.组织样本前处理：取20 mg组织样本放入1.5 mL离心管（自备）中，加入500 μL的 Buffer RLC，组织匀浆仪打碎后，12000 rpm（~13400×g）离心1分钟，取200 μL 上清为样本。
操作步骤
1.取1.5 mL离心管（自备），加入500 μL Buffer RLC，200 μL样本，20 μL Proteinase K，涡旋震荡5 s后，置于室温，1200 rpm的恒温混匀仪震荡10 min。 注：湿拭子样本，充分震荡混匀后取200 μL进行提取。干拭子样本浸泡于400 μL生理盐水中，充 分震荡混匀后静置5分钟，12,000 rpm离心1分钟后，取200 μL进行提取。
2.瞬离离心管，将步骤1所得溶液加入到已装入收集管的吸附柱（Spin Columns DM） 中。12,000 rpm（~13,400×g）离心1分钟，倒掉收集管中的废液，将吸附柱重新 放回收集管中。
3.向吸附柱中加入500 μL Buffer PGWT，12,000 rpm离心1分钟，倒掉收集管中的废 液， 将吸附柱重新放回收集管中。
4.向吸附柱中加入500 μL Buffer GWT2，12,000 rpm离心1分钟，倒掉收集管中的废 液， 将吸附柱重新放回收集管中。 1
5.2,000 rpm离心2分钟，倒掉收集管中的废液。将吸附柱置于室温2分钟，晾干。
6.将吸附柱置于新的收集管（RNase-Free Centrifuge Tube）中，向吸附柱膜的中间 部位悬空加入40-100 μL RNase-Free Water，室温放置2分钟，12,000 rpm离心1 min， 收集核酸溶液。置于-80℃长期保存。
Products content
Products Introduction
This kit is suitable for simple, rapid and efficient isolation and purification of DNA/RNA from whole blood, tissue homogenates, swabs, serum, plasma and other cell-free body fluids, etc. The unique buffer system enables the viral nucleic acids in the lysate to bind to the silica gel centrifugal adsorbent columns in a highly efficient manner, and the viral nucleic acids obtained are of high purity and stable quality, free of protein, nuclease and other impurities, and can be used in a variety of routine operations, including PCR, fluorescence quantitative PCR and other experiments. It can be used for a variety of routine operations, including PCR, fluorescence quantitative PCR and other experiments.
Bring your own instruments
Thermostatic mixer.
Pre-experiment Preparation and Important Notes
1. Read these instructions carefully before experimenting.
2. If Proteinase K is to be stored for a long period of time, please keep it at -20℃.
3. Check Buffer RLC for crystallization or precipitation prior to use, and if crystallization or precipitation occurs, redissolve Buffer RLC in a 56°C water bath.
4. Pre-treatment of tissue samples: Take 20 mg of tissue samples into 1.5 mL centrifuge tubes (self-provided), add 500 μL of Buffer RLC, and after the tissue homogenizer breaks up, centrifuge the samples for 1 minute at 12,000 rpm (~13,400×g), and take 200 μL of supernatant as samples.
procedure
1. Take a 1.5 mL centrifuge tube (provided), add 500 μL of Buffer RLC, 200 μL of sample, 20 μL of Proteinase K, vortex for 5 s, and then place it in a thermostatic mixer at 1200 rpm for 10 min at room temperature. Note: For wet swab samples, 200 μL of sample was taken after sufficiently shaking and mixing. Note: For wet swabs, 200 μL was taken from the sample after it was soaked in 400 μL of saline, shaken and mixed thoroughly for 5 minutes, and then centrifuged at 12,000 rpm for 1 minute, and 200 μL was taken for extraction.
2. Instantly remove the centrifuge tube and add the solution from step 1 to the Spin Columns DM in the collection tube. centrifuge at 12,000 rpm (~13,400 x g) for 1 minute, pour off the waste liquid from the collection tube, and return the column to the collection tube.
3. Add 500 μL of Buffer PGWT to the adsorbent column, centrifuge at 12,000 rpm for 1 minute, pour off the waste liquid from the collection tube, and return the column to the collection tube.
4. Add 500 μL of Buffer GWT2 to the adsorbent column, centrifuge at 12,000 rpm for 1 minute, pour off the waste liquid from the collection tube, and return the column to the collection tube.
5. Centrifuge at 12,000 rpm for 2 minutes and pour off the waste liquid in the collection tube. Place the adsorption column at room temperature for 2 minutes and allow to dry.
6. Place the column in a new collection tube (RNase-Free Centrifuge Tube), add 40-100 μL of RNase-Free Water to the center of the column membrane, let it stand at room temperature for 2 minutes, and then centrifuge at 12,000 rpm for 1 minute to collect the nucleic acid solution. Store at -80℃ for a long time.
规格
英文名称
Viral DNA/RNA Kit V2
储存与运输
储存条件
室温
运输条件
常规运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于室温条件下保存。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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