Eliminates the need to waste precious samples on repeated gel electrophoresis for detecting different primary antibodies. Simply treat the membrane with this stripping buffer to remove the first primary antibody. This process typically takes 10–20 minutes, depending on the amount of antibody bound to the membrane. After stripping, the membrane can be reprobed with a new primary antibody.
Key Features
Saves samples: The same membrane with identical samples can be used for multiple detections.
Time-efficient: No need to rerun gel electrophoresis.
Highly effective: Superior stripping performance compared to homemade buffers.
Gentle formulation: Minimal damage to target proteins on the membrane.
Odorless: No pungent odor, more environmentally friendly.
Protocol
After completing Western Blot chemiluminescence detection, rinse the membrane with TBS/T or PBS/T for 5 minutes.
Add 10–20 mL of stripping buffer to cover the membrane. Incubate on a shaker for 10–20 minutes (duration depends on the amount of antibody bound).
Discard the stripping buffer and rinse the membrane with TBS/T or PBS/T for 5 minutes.
Optional: Apply chemiluminescence substrate to quickly verify stripping efficiency.
Reblock the membrane with an appropriate blocking buffer before reprobing with other antibodies.
The membrane can be stripped at least 10 times without significantly affecting signal intensity.
Example: GAPDH signals showed no significant reduction after 6 hours of continuous stripping (equivalent to 24 cycles at 15 min each).
规格
规格或纯度
BioReagent, 即用型, 适用于电泳, for western blot
稳定性与储存
Store at 2-8℃ long term (12 months).
英文名称
Western Blot Fast Stripping Buffer
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
图片
Stripping Efficiency of the WB Antibody Rapid Stripping Buffer (Mild, Universal Type) (W1491287)
Experimental Method:
The same PVDF membrane was sequentially probed with antibodies against different target proteins, with antibody stripping performed using this product in between.
Results:
Before stripping (left): Immunoblotting with Recombinant CEP55 Antibody (Ab336940) (target molecular weight ~55 kDa) showed a clear and specific band, indicating efficient primary antibody binding.
After stripping (right): Following a 15‑minute incubation with this product at room temperature, the same membrane was re‑probed with Recombinant Hsp70 Antibody (Ab108909) (target molecular weight ~70 kDa). A distinct, strong band for HSP70 was observed, with a clean background and no residual signal from the previous detection.
ZJ25F0927432
危险品化学品经营许可证（带存储）