计算溶液所需的质量、体积或浓度。
BioReagent,适用于植物细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
WPM(Woody Plant Medium,木本植物培养基)由 Lloyd 与 McCown 开发,专用于木本植物离体培养。与 MS 培养基相比,其总盐和铵离子浓度大幅降低,更契合木本植物的营养需求。
本品为 WPM 的定制改良版,去除原配方中的硝酸钙,干粉推荐用量为 2.14 g/L。该设计可有效避免钙离子过量沉淀及硝酸盐胁迫等不利影响,适用于钙调控、钙胁迫等特殊实验体系。产品广泛应用于木本植物的芽增殖、愈伤组织诱导、继代培养及生根等组织培养研究。本产品不含硝酸钙,配制培养基时需由使用者按实验方案自行补加钙源,否则钙离子缺失会造成植物生长异常。仅用于科研领域,不可用于食品、临床诊断。
产品成分:
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使用方法:
1、准确称取本品 2.14g, 另取硝酸钙 0.56g,加入 1L 蒸馏水中,直至粉末完全溶解,配制成均匀培养基溶液。
2、采用 116℃ 高压蒸汽灭菌 30 分钟,灭菌完成后置于洁净环境自然冷却,即可用于植物组织培养实验。
注意事项:
1、pH 值根据需求进行调整。
2、建议用室温水溶解后再高压灭菌,不建议加热溶解。
3、室温,避光保存,有效期 3 年。
4、植物组织培养基,全无机盐配方,灭菌后有少量沉淀。
5、本产品仅供科研使用,请勿用于医药、临床诊断或治疗,食品及化妆品等用途,请勿存放于普通住宅区。
6、本公司将不为任何不正常使用此产品时所发生的意外负责。
WPM (Woody Plant Medium) was developed by Lloyd and McCown specifically for the in‑vitro culture of woody plants. Compared with MS medium, it has substantially reduced total‑salt and ammonium‑ion concentrations, which better matches the nutritional requirements of woody plants.
This product is a custom‑modified version of WPM with calcium nitrate removed from the original formula. The recommended powder dosage is 2.14 g/L. This design effectively prevents adverse effects such as excessive calcium‑ion precipitation and nitrate stress, making it suitable for special experimental systems including calcium modulation and calcium‑stress studies. It is widely used in tissue‑culture research of woody plants for shoot proliferation, callus induction, subculture and rooting. This product does not contain calcium nitrate. Users must supplement calcium sources according to their experimental protocols when preparing the medium; otherwise, calcium deficiency may lead to abnormal plant growth. For research use only. Not for use in food products or clinical diagnostics.
Product Composition:
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Instructions for Use:
1. Accurately weigh 2.14g of this product and 0.56g of calcium nitrate, add both to 1L of distilled water, stir until the powders are fully dissolved to prepare a homogeneous culture medium solution.
2. Sterilize the solution via autoclaving at 116℃ for 30 minutes. After sterilization, let it cool naturally in a clean environment, and the medium is then ready for plant tissue culture experiments.
Precautions:
1. The pH value shall be adjusted as required.
2. Dissolve the powder in room-temperature water prior to autoclaving; heating for dissolution is not recommended.
3. Store at room temperature away from light; shelf life is 3 years.
4. This plant tissue culture medium adopts a full inorganic salt formula. Minor precipitation may occur after sterilization.
5. This product is for research use only. Do not use it for pharmaceutical purposes, clinical diagnosis or treatment, food production, cosmetics manufacturing or other related applications. Do not store it in residential areas.
6. Our company shall not be liable for any accidents arising from improper use of this product.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | W1524322 |
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