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铵根离子（NH₄⁺）含量是评价水质是否受到污染的重要指标之一。水中铵根离子以游离氨（NH₃）或铵盐（NH₄⁺）形式存在，两者比例主要取决于水样的pH值。本试剂盒采用靛酚蓝比色法测定铵根离子含量：在强碱性介质中，铵根离子转化为铵盐形式，与苯酚和次氯酸钠反应生成稳定的水溶性靛酚蓝染料，该产物在625 nm处具有特征吸收峰。通过测定该波长下吸光值，吸光度与铵根离子含量成正比，结合标准曲线即可精准定量样品中的铵根离子浓度。
The ammonium ion (NH₄⁺) content is one of the important indicators for evaluating whether water quality is contaminated. In water, ammonium ions exist in the form of free ammonia (NH₃) or ammonium salts (NH₄⁺), and their ratio mainly depends on the pH value of the water sample. This kit employs the indophenol blue colorimetric method for the determination of ammonium ion content: in a strongly alkaline medium, ammonium ions are converted to the ammonium salt form, which reacts with phenol and sodium hypochlorite to generate a stable water-soluble indophenol blue dye. This product exhibits a characteristic absorption peak at 625 nm. By measuring the absorbance at this wavelength, which is proportional to the ammonium ion content, and combining with a standard curve, the ammonium ion concentration in the sample can be precisely quantified.
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