UltraBio™ Western检测增敏剂是一种蛋白印迹的一抗或酶标二抗通用稀释液，可增强检测实验中 抗原抗体间的结合，尤其在用于目的蛋白丰度极低或抗体效价较低而导致目的蛋白条带信号弱的情况，可以使western blot 检测的灵敏度增强数倍,从而有效解决检测过程中经常遇到 的低灵敏度等问题。本产品也可用于酶联免疫吸附分析法(ELISA)､免疫斑点印迹(Dot-Blot)､ 免疫荧光(Immuno-Fluorescence)､免疫组化(Immuno-Histochemistry)等免疫学检测的增敏。
缓冲液 Buffer：MOPS
蛋白 Protein：BSA
保质期 Shelf life：2 years
储存 Storage：2-8℃
应用 Application：Western blot
产品特点：
1、显著提高目的条带的信号强度：增强检测实验中抗原抗体间的结合，尤其在用于目的蛋白丰度极低或抗体效价较低时。对于较难检测的蛋白，可节省检测优化的时间和精力。
2、易获得更理想的检测结果：本产品使目的条带信号更强，较低的背景，检测结果更佳。
3、可节省抗体使用量：可以适当减少一抗和二抗的用量，有效节约实验成本。同时，本产品稀释和配制的一抗或二抗可以 2-8℃保存，可以反复多次用于 Western blot。
4、使用简单方便：本产品是一种即用型溶液，通用于一抗和二抗的稀释，不增加或改变常规的 Western 检测步骤。
5、通用及适用性好：本产品兼容 PVDF 膜和 NC 膜，也可用于 ELISA、斑点印迹、免疫荧光、免疫组化等免疫检测。
注意事项：
1、稀释配制好的一抗或二抗使用次数较多后会出现检测效果下降，如出现检测效果下降、浑浊、沉淀等情况宜丢弃。为了使稀释的一抗或二抗可以多次使用，抗体孵育结束后应立即回收稀释的抗体并立即存放在 2-8℃。
2、产品仅用于一抗或二抗的稀释，不可用于印迹膜的封闭，也不可用于 ELISA 检测中抗原或抗体的包被。
3、产品无须再添加其它的封闭试剂如 BSA、脱脂奶粉、酪蛋白等。如果额外添加其它试剂，可能带来负面效果。
4、由于目的蛋白和抗体的不确定性，本产品有可能对某些目的蛋白和相应抗体的检测信号强度的提高效果一般。
5、本产品实际使用过程中，如果目的条带信号非常强的情况下，非特异性条带的信号也会相应地有所增强。此时可以尝试适当降低蛋白上样量、降低一抗二抗浓度或缩短曝光时间。
UltraBio™ Western Blot Enhancer is a universal dilution buffer for primary antibodies or enzyme-labeled secondary antibodies in Western blotting. It can enhance the binding between antigens and antibodies in detection experiments, especially when the target protein band signal is weak due to extremely low abundance of the target protein or low antibody titer. It can increase the sensitivity of Western blot detection several times, thus effectively solving problems such as low sensitivity often encountered in the detection process. This product can also be used for sensitization in immunological detection methods such as Enzyme-Linked Immunosorbent Assay (ELISA), Dot-Blot, Immuno-Fluorescence, and Immuno-Histochemistry.
Buffer: MOPS
Protein: BSA
Shelf Life: 2 years
Storage: 2-8℃
Application: Western blot
Product Features:
- Significantly Enhance Target Band Signal Intensity: It enhances the binding between antigens and antibodies in detection experiments, especially when the target protein abundance is extremely low or the antibody titer is low. For proteins that are difficult to detect, it can save time and effort in detection optimization.
- Easy to Obtain More Ideal Detection Results: This product makes the target band signal stronger with lower background, resulting in better detection results.
- Save Antibody Usage: The dosage of primary and secondary antibodies can be appropriately reduced, effectively saving experimental costs. Meanwhile, the primary or secondary antibodies diluted and prepared with this product can be stored at 2-8℃ and can be used repeatedly for Western blot.
- Simple and Convenient to Use: This product is a ready-to-use solution, universally applicable for the dilution of primary and secondary antibodies, without adding or changing the conventional Western detection steps.
- Good Universality and Applicability: This product is compatible with PVDF membranes and NC membranes, and can also be used in immunoassays such as ELISA, Dot-Blot, Immuno-Fluorescence, and Immuno-Histochemistry.
Precautions:
- The detection effect of the diluted primary or secondary antibodies will decrease after multiple uses. If the detection effect decreases, or there is turbidity, precipitation, etc., they should be discarded. To enable the diluted primary or secondary antibodies to be used multiple times, the diluted antibodies should be recovered immediately after the antibody incubation and stored at 2-8℃ immediately.
- This product is only used for the dilution of primary or secondary antibodies, not for the blocking of blotting membranes, nor for the coating of antigens or antibodies in ELISA detection.
- There is no need to add other blocking reagents such as BSA, skimmed milk powder, casein, etc. to this product. Adding other reagents extra may lead to adverse effects.
- Due to the uncertainty of target proteins and antibodies, this product may have a general effect on increasing the detection signal intensity of some target proteins and corresponding antibodies.
- In the actual use of this product, if the target band signal is very strong, the signal of non-specific bands will also be enhanced accordingly. At this time, you can try to appropriately reduce the protein loading amount, lower the concentration of primary and secondary antibodies, or shorten the exposure time.
ZJ25F1129860
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