本产品适用于化学发光法检测和荧光检测的Western Blot实验，可对已完成检测的硝酸纤维素膜或PVDF膜进行重复利用。在室温条件下，可在不影响膜上蛋白的情况下，去除膜上的一抗和二抗，无需反复电泳和转膜，节省样品和时间。
本产品配方温和，操作简单快速，可对同一张膜进行3-5次的Western Blot检测。
产品编号 | W751794 | W751792 | W751795 | 产品名称 | 蛋白印迹膜再生液（酸性） | 蛋白印迹膜再生液（强碱性） | 蛋白印迹膜再生液（中性） | 产品包装 | 100mL, 500mL | 100mL, 500mL | 100mL, 500mL | pH | 酸性（2.0-2.3） | 强碱性（12.5-13.5） | 中性（7.0-8.0） | 去除一抗二抗所需时间 | 10-30分钟 | 20-50分钟 | 10-20分钟 | 去除一抗二抗的效果 | 好 | 好 | 好 | 使用膜的类型 | PVDF和NC | PVDF和NC | PVDF和NC | 有无刺鼻性气味 | 无气味 | 无气味 | 无气味 |
三款膜再生液均是多种抗体去除试剂的复合配方，强碱性配方，去除效果稍弱，腐蚀性强，但性价比高。中性和酸性膜再生液，抗体去除能力相近，腐蚀性弱，均可使用。温度过低会降低去除效果，可在使用前适当提高膜再生液温度再使用。 |
注意事项：
1、如果在使用前溶液出现沉淀，可37°C加热5min以促进溶解。
2、建议先检测表达量较低的目的蛋白，之后用再生液处理并检测表达量高的蛋白。
3、不推荐使用BSA封闭印迹膜，以及DAB，NBT/BCIP显色的印迹膜。
使用说明：
1、将检测后的膜从机器内取出，用PBST或TBST清洗10分钟，弃清洗液。
2、用再生液进行洗脱，洗脱时间的长短主要取决于蛋白质表达量、抗体类型和浓度。如果检测信号不强，可室温振荡洗脱30-60分钟；如果是表达量高、信号强的样品，可按其使用体积，加入终浓度20mM DTT或100mM β-巯基乙醇，室温震荡洗脱30-60分钟。
3、弃去再生液，用适量体积PBST或TBST漂洗3次，每次5分钟。
4、此时膜上的抗体已被洗脱，可重新封闭，进行下一轮检测。
This product is suitable for Western Blot experiments of chemiluminescence and fluorescence detection, and can be reused on nitrocellulose or PVDF membranes that have been tested. At room temperature, primary and secondary antibodies on the membrane can be removed without affecting the protein on the membrane, without the need for repeated electrophoresis and membrane transfer, saving samples and time.
This product has a mild formula, simple and fast operation, and can perform 3-5 Western Blot tests on the same membrane.
Precautions：
1. If precipitation occurs in the solution before use, it can be heated at 37 ° C for 5 minutes to promote dissolution.
2. It is recommended to first detect the target protein with low expression levels, and then treat it with regeneration solution and detect the protein with high expression levels.
3. It is not recommended to use BSA sealed imprinting membranes, as well as DAB, NBT/BCIP stained imprinting membranes.
Instructions for Use：
1. Remove the tested membrane from the machine, clean it with PBST or TBST for 10 minutes, and discard the cleaning solution.
2. The duration of elution using regeneration solution mainly depends on the protein expression level, antibody type, and concentration. If the detection signal is not strong, it can be eluted by shaking at room temperature for 30-60 minutes; If it is a sample with high expression and strong signal, 20mM DTT or 100mM β - mercaptoethanol can be added according to its usage volume, and shaken at room temperature for 30-60 minutes for elution.
3. Discard the regeneration solution and rinse 3 times with an appropriate volume of PBST or TBST, each time for 5 minutes.
4. At this point, the antibodies on the membrane have been eluted and can be resealed for the next round of testing.
ZJ25F0420215
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