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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
黄嘌呤与次黄嘌呤在黄嘌呤氧化酶(XOD)作用下生成过氧化氢(H₂O₂)。过氧化物酶(POD)催化H₂O₂氧化4-氨基安替比林,使其与TOOS发生偶联反应,生成稳定的紫红色醌亚胺化合物。该产物在550 nm处具有特征吸收峰,通过测定该波长下吸光度的变化,即可定量检测样品中黄嘌呤与次黄嘌呤的含量。
Under the action of xanthine oxidase (XOD), xanthine and hypoxanthine generate hydrogen peroxide (H₂O₂). Peroxidase (POD) catalyzes the oxidation of 4‑aminoantipyrine by H₂O₂, which then undergoes a coupling reaction with TOOS to form a stable purple‑red quinone imine compound. This product exhibits a characteristic absorption peak at 550 nm. By measuring the change in absorbance at this wavelength, the content of xanthine and hypoxanthine in the sample can be quantitatively determined.